Introduction
Protein post-translational modifications (PTMs) refer to chemical modifications of proteins during or after
translation, which play key roles in many cellular processes, such as cell differentiation, protein degradation,
signaling and regulatory processes, gene expression regulation, and protein interaction. Common PTMs include
phosphorylation, acetylation, ubiquitination, glycosylation and so on, which add a layer to the proteome by adding
biochemical moieties to specific residues of a protein, altering its structure, function, and/or localization
complexity. Therefore, the characteristics of PTMs, including modification classes and modification sites, are
crucial in cell biology as well as disease diagnosis and prevention research. Currently, mass spectrometry
(MS)-based techniques are at the forefront of PTMs analysis, capable of detecting a large number of modified
proteins with high levels of sensitivity and specificity.
Fig. 1. Post-translational
modifications (PTMs) within the mammalian cell. (Dunphy K, et al., 2021)
Our Services
Creative Proteomics, a leading global provider of protein sequencing, provides high-quality PTMs
analysis using mass spectrometry (MS)-based methods. Here, we have built an advanced analytical platform that can be
used to characterize various protein translations. We offer mass spectrometry-based methods that provide high
reproducibility, sensitivity, specificity, and accurate mass data.
Protein post-translational modifications (PTMs) are affected by many factors. The levels of most post-translational
modifications are very low and must be enriched before identification. Creative Proteomics provides
comprehensive PTMs analysis services to meet diverse needs by providing bottom-up and top-down approaches.
In the identification process of bottom-up protein post-translational modification, the protein will first be cut
into peptide fragments by enzymes, then analyzed by mass spectrometry, and the molecular mass information of a
series of peptide fragments is obtained. The top-down approach is to directly analyze the entire protein.
Creative Proteomics provides the following protein post-translational modification identification
analysis services but not limited to:
*Note: We also offer custom analysis services to identify many other types of PTMs. For special requirements, please
contact us for project discussion.
With years of experience in protein sequencing, Creative Proteomics provides a one-stop-shop for
protein PTMs analysis to customers worldwide, including:
- Sample preparation and pretreatment.
- LC-MS/MS identification.
- Peptide identification.
- Data analysis.
- Protein PTM site assessment.
- Detailed formal report.
Which Sample Types Can be Analysed?
- Plant Tissue Samples
- Animal Tissue Samples
- Blood Samples
- Urine
- Cellular Content
- Cerebrospinal Fluid
- Protein Samples
- Additional Sample Types on Request
Creative Proteomics is a trusted provider of proteomics analysis services. Thanks to our outstanding
scientists and powerful mass spectrometry platform, we offer a range of PTMs analysis services to our clients
worldwile. Our services guarantee accurate and reliable results, at quick turnaround time! If you are interested in
our services, please contact us to see how we can help solve your problem.
References
- Dunphy K, Dowling P, Bazou D, et al. (2021) Current methods of post-translational modification analysis
and their applications in blood cancers. Cancers (Basel). 13(8):1930.
- Larsen M R, Trelle M B, Thingholm T E, et al. (2006) Analysis of posttranslational modifications of
proteins by tandem mass spectrometry. Biotechniques. 40(6):790-798.
