What is biological assay?
Biological assay is generally used to determine the biological activity of the substance, such as biological drugs, by using animals, tissue or cells. This assay has two main purposes for pharmacokinetic analysis. It is typically conducted to evaluate the potency of the drug before manufacturing. A biological assay also can be used to measure the concentration of the drug in body fluid.
What is in vivo assay?
Biological assays can be divided into in vivo assay and in vitro assay. During in vivo assay, the animals are required for administration of biological pharmaceutical products. The biological activity and effect of drugs can be measured on a living organism. Animal's physiological responses to biological drugs may predict patients' responses. Thus in vivo biological assay is one of the most direct methods for pharmacokinetics analysis. However, time consuming, low sensitivity and variation make it less useful compared with in vitro assay.
What is in vitro assay (cell-based assay)?
In vitro biological assay usually uses cell lines or tissues that respond to biological drugs. Cell-based assay has become the most useful method for biological assay depending on the cell lines with high sensitivity and stability. There are various cell-based assays due to different mechanisms of the interaction between protein drugs and cells.
Scientists from Creative Proteomics are professional in cell biology and able to offer various cell-based assays for pharmacokinetic assays. Creative Proteomics provides a wide range of cell-based assay, including:
- cell proliferation assay
- cell death assay
- cell metabolism and protein turnover
- other cell-based assays
