Vaccines refer to biological products made with various pathogenic microorganisms for vaccination. Vaccines are autoimmune preparations made of pathogenic microorganisms (such as bacteria, rickettsiae, viruses, etc.) and their metabolites through artificial attenuation, inactivation, or genetic modification to prevent infectious diseases. The development of vaccines has undergone three revolutions. The first generation is traditional inactivated vaccines and attenuated vaccines, the second generation is recombinant protein vaccines, and the third generation is nucleic acid vaccines.
Recombinant protein vaccines are gene sequences that can express viral surface antigens (S or S1), which are transferred into prokaryotes by means of genetic engineering, so that they can express antigen proteins in large quantities. Then the expressed antigen protein is extracted and purified for vaccination. The development of recombinant vaccines requires a process of protein construction, expression, purification and identification. In order to ensure the safety and effectiveness of vaccines, strict identification of vaccine proteins must be implemented in discovery, development, and quality assessment.
Creative Proteomics has a professional analysis platform that can provide you with vaccine protein identification services. We are committed to becoming the most reliable ally in the development of biological vaccines, providing you with accurate and comprehensive product analysis reports. We will provide you with vaccine protein identification and analysis services in accordance with industry guidelines or the laws and regulations of various countries and regions.
We Can Provide but Not Limited to:
| Service | Method |
| Vaccine Protein Molecular Weight Assay | ESI-QTOF-MS |
| MALDI-TOF-MS | |
| Vaccine Protein N-Terminal Sequencing | Edman degradation; Triple quadrupole tandem mass spectrometry |
| Vaccine Protein C-Terminal Sequencing | MALDI-ISD MS |
| Vaccine Protein Peptide Mapping | MALDI-TOF-MS; Triple quadrupole LC-MS/MS |
| Vaccine Protein Amino Acid Analysis | High performance liquid chromatography (HPLC); Thin layer chromatography (TLC) |
| Vaccine Protein Isoelectric Point Assay | Isoelectric focusing (IEF) |
| Capillary isoelectric focusing (cIEF) |
Technology Platform of Vaccine Protein Identification Service:
In vaccine development and quality control, Creative Proteomics utilizes high-resolution mass spectrometry, liquid phase mass spectrometry, and capillary electrophoresis techniques to effectively and accurately identify and analyze the physical and chemical properties of vaccine proteins.
1) Mass spectrometry
The ultra-high resolution LC/MS instrument has excellent resolution, sensitivity, dynamic range and quality accuracy, and can analyze vaccine proteins more quickly and accurately.
ESI Q-TOF mass spectrometry analysis
MALDI-TOF mass spectrometry analysis
Triple quadrupole tandem mass spectrometry
2) Capillary electrophoresis
Capillary electrophoresis is mainly based on mass number, charge and molecular shape for separation. Depending on the detector, there are many methods, from CE/UV or CE/LIF (laser induced fluorescence detection) to CE/LIF/MS, a combination scheme combining laser induced fluorescence detection and mass spectrometry detection.
Advantages of Vaccine Protein Identification Service:
➢ Short time-consuming: Mature technology and advanced equipment accelerate the progress of the project.
➢ Advanced technology platform: Equipped with high-resolution mass spectrometry, liquid mass spectrometry and other equipment.
➢ Rapid turnaround time: 5-7 days can provide customers with accurate and comprehensive experimental reports.
➢ Customized service: We can provide you with tailor-made services based on your needs, and you can also choose your own analysis solutions.
Creative Proteomics has a professional biological vaccine service provider, especially in the field of vaccine characterization related analysis. We can provide you with high-quality experimental analysis reports, including comprehensive and accurate experimental design plans, detailed data, maps and results interpretation reports. We look forward to working with you.
