Nowadays, purified proteins have been used for therapeutic and diagnostic applications more and more frequently, when worked with proteins in the lab, whether the proteins are of poor quality or impure, bad samples can lead to inaccuracy and low precision across experiments, sometimes may cause experiments to fail completely.
There are a large number of methods that can measure protein quantity and purity. Generally, we can check the purity by quantification methods like UV-Vis, Bradford and Activity Assays. Meanwhile, electrophoresis is widely used by biochemists and can provide a general picture of both the size of your target protein whether other protein-based impurities present. Such as SDS-PAGE, one method that can separates proteins and proceed further with CBB (Coomassie Brilliant Dyes) or silver staining.
Mass spectrometry is used more often in recent years, a very powerful analytical technique with great precision and accuracy that can identify post-translational modifications, it is not easily visualized with the techniques described above. Dynamic Light Scattering, a widely used method for assessing homogeneity. uses polarized laser light to measure the level of diffraction in small molecule samples. Microfluidic Diffusional Sizing (MDS), MDS exploits the unique properties of flow in microfluidic channels – specifically laminar flow, where streams can flow alongside one another with no convective mixing. High performance liquid chromatography is a technique that includes separation, identification and quantification, used for compounds in analyses.
The above techniques that we described can provide the protein samples a comprehensive and accurate picture with the use of one another.
Creative Proteomics offers a well-established analytical platform. Our full-scale service package contains various analytical items to meet your requirements.