Venom Peptide Qualification

In animal venom, most of the biologically active ingredients with pharmacological significance are peptides, that is, polypeptides with a molecular weight of less than 10 kDa. These active peptides have powerful biological functions. Peptide analysis of animal venom is a new and promising scientific field.

The quantification of small differences in peptide content between samples is one of the most important and challenging tasks in peptomics. Quantitative research on venom peptideomics generally includes labeled (isotopic and isobaric) and label-free quantification methods.

Creative Proteomics relies on an advanced omics research platform and a professional research team to help you carry out quantitative research on venom peptide omics.

Label-free Quantification

Label-free quantification is a mass spectrometry analysis of peptides through liquid-mass spectrometry technology. The quantification of unlabeled peptides can be performed by extracting the peptide signal intensity or by spectral counting. The ion signal intensity approach uses the extracted chromatographic area to compare peptide abundances across samples. Spectral counting totals the number of times a peptide is selected for fragmentation and identified in a data-dependent acquisition experiment. This method does not need to use expensive stable isotope labels as internal standards, requires a small amount of samples, and has low cost.

Isobaric Labeling

Isobaric labeling is a mass spectrometry strategy used in quantitation. Peptides are labeled with various chemical groups that are (at least nominally) identical masses (isobaric), but vary in terms of distribution of heavy isotopes around their structure. These tags, commonly referred to as tandem mass tags, are designed so that the mass tag is cleaved at a specific linker region upon high-energy CID (HCD) during tandem mass spectrometry yielding reporter ions of different masses. Isobaric labeling has been successfully used for many biological applications.

Isotope Labeling

Isotope dilution mass spectrometry avoids the influence of matrix effects and analysis conditions, has special advantages in quantitative analysis and accuracy, and has been widely used in the field of quantitative analysis of biomolecules. Stable isotope-labeled peptides are widely used as important internal standard reagents in dairy product detection, biological protein quantification, protein sequencing, drug metabolism, and food allergen detection. This method has the advantages of high throughput, good repeatability, accurate quantification, reliable identification results, and simple operation.

Creative Proteomics is an expert in venomics research. We can provide you with customized research solutions and one-stop research services.

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