Integrated Analysis of Transcriptomics and Metabolomics
Introduction
The transcriptome is a collection of complete transcription information in a cell under specific developmental stages or physiological conditions, and represents the intermediate state of gene expression. Metabolomics is an emerging discipline in the field of systems biology. It explores the metabolic mechanism of the entire organism by detecting changes in the metabolites in the organism after exogenous stimulation or genetic modification. Metabolomics is the large-scale study of small molecules in cells, biological fluids, tissues, or organisms. A comprehensive analysis of a series of metabolites produced in the life process of an organism helps to reveal the connection between genotype and phenotype. Integrated multi-omics analysis is currently the most effective method for comprehensive analysis of metabolites.
The integrated analysis of venom transcriptomics and metabolomics refers to the normalization and statistical analysis of batch data from the venom transcriptome and metabolome, and the establishment of data relationships between molecules of different levels (such as mRNA, metabolites). At the same time, it combines functional analysis, metabolic pathway enrichment, molecular interaction and other biological function analysis to systematically and comprehensively analyze the functions and regulation mechanisms of venom, and finally realize a comprehensive understanding of the general trends and directions of biological changes. The integrated analysis of transcriptomics and metabolomics for toxic species or venom can analyze the internal changes of organisms (such as the synthesis of venom) from two levels of cause and effect. In order to analyze the molecular function and regulation mechanism of venom more systematically and comprehensively, as well as the synthesis mechanism of venom and the evolution process of venom.
What Creative Proteomics Offers
Creative Proteomics' transcriptome sequencing service based on the Illumina high-throughput sequencing platform can detect the overall transcription level of any species at the single nucleotide level. Metabolomics analysis services based on GC-MS, LC-MS, nuclear magnetic resonance and other technical means can realize targeted and non-targeted metabolomics analysis of a variety of samples. In addition, it also provides customizable bioinformatics analysis methods for integrated analysis. Creative Proteomics provides you with a one-stop integrated analysis service of venom transcriptomics and metabolomics from experimental design, high-throughput sequencing, to biological information data analysis, to meet different testing needs.
Method Flow
Fig 1. Transcriptomics and metabolomics integrated analysis pipeline - Creative Proteomics
Integrate Analysis Content
- Multivariate statistical joint analysis of venom transcriptome and metabolome: O2PLS association analysis, OPLS related variables screening.
- Venom transcriptome and metabolome KEGG metabolic pathway joint analysis: KEGG pathway visualization, pathway comparison analysis, enrichment analysis.
- Venom transcriptome and metabolome correlation analysis: correlation coefficient matrix heat map, correlation analysis hierarchical clustering heat map, correlation coefficient regulation network analysis.
- Comparative analysis of PCA between venom transcriptome and metabolome (limited to ≥3 groups).
Solved Problem
Through the integrated analysis of venom transcriptomics and metabolomics, the following problems can be solved:
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Through the analysis of expression levels at different levels, the full spectrum analysis of venom gene expression and metabolites is realized.
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Explore biological issues from the two directions of gene expression and metabolites, and conduct mutual verification. Screen out important metabolic pathways, genes, or metabolites to provide a data basis for subsequent in-depth experimental analysis and applications.
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Through transcriptomics and metabolomics research, based on correlation analysis, construct a gene-metabolite regulatory network, screen key genes such as venom synthesis, and lay the foundation for venom research.
Project Report
In the result report, Creative Proteomics will provide you with a detailed technical report, including:
- Experimental procedure (such as NGS and LC-MS/MS).
- Related experimental parameters.
- The raw sequencing data, including transcriptomics data and metabolomics data.
- Software and parameter settings used in the analysis process.
- Data analysis result pictures and tables.
- Interpretation of results.
How to Order?
Creative Proteomics provides customers with professional integrated analysis of transcriptomics and metabolomics data analysis services, aiming to help you understand the venom from the mRNA and metabolites level. In addition, we also provide other different types of multi-omics joint analysis services. For high-throughput sequencing and integrated analysis of transcriptomics and metabolomics bioinformatics data analysis, if you have any questions, please feel free to contact us. Look forward to working with you！
For research use only. Not intended for any clinical use.
