Frog Venom Solutions

Certain species of frogs have now been identified as poisonous frogs. Although frogs do not have a tube to transport the poison produced in the poisonous glands to the victim, their venom is also very potent, such as Bruno casque-headed frog, Greening's frog, etc. The venom of frogs is secreted from the skin and is also a rich source of bioactive compounds such as biogenic amines, alkaloids, bufadinol, steroids, lipids, peptides and proteins.

Creative Proteomics provides solutions for frog venom to help you study the composition and characterization of frog venom, biological effects and its application.

Our Frog Venom Solutions

We use mass spectrometry and chromatography to analyze your frog venom sample, study the protein/peptide in the venom and evaluate its biological activity, such as hemolysis, phospholipase A₂ activity, coagulation activity, proteolytic activity and amide hydrolysis activity. If you want to explore the toxicity of frog venom, we also provide venom cytotoxicity and morphological analysis services.

Mass Spectrometry and Chromatographic Analysis

• Sample processing. The frog venom is centrifuged and extracted.
• MS analysis. The samples were analyzed by matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF/MS). The sample is pre-mixed with the matrix solution, the mixture is applied to the sample holder for automatic analysis, and the software is used for data collection and processing.
• RP-HPLC analysis. The sample is applied to the chromatographic column, and the chromatographic column is eluted at a constant flow rate for UV detection.
• GC-MS analysis. Gas chromatography and mass spectrometry are coupled to obtain sample mass spectra, and the results are compared with databases to determine the identity of the molecule.

Analysis of Biological Activity in Frog Venom

We analyze the biological activity characteristics of frog venom, and mainly use absorbance methods such as microplate reader to detect related activities, including but not limited to the following biological activity indicators.

We also provide frog venom toxicity testing, using tissue cells to check the venom cytotoxicity, reading the absorbance of the released dye and calculating the percentage of cell viability. The morphological changes and cell damage were assessed qualitatively by light/phase contrast microscopy. Quantitative data analysis uses one-way analysis of variance for statistical comparison and testing.

Sample Requirements

• If you have prepared a sample of frog venom, please freeze-dry the sample and submit 1mg.
• If you don't have a ready-made frog venom sample, we provide a sample collection service. The venom is obtained by immersing the target species in ultrapure water and irritating the skin by rubbing the entire body, and freeze-dried venom is stored at -70°C.

Advantages

Creative Proteomics provides high-precision, high-throughput, high-range, complete and comprehensive low-cost frog venom solutions. The analysis of the components of frog venom and the exploration of its biological activity are of great significance, and its application in the treatment of venom and disease has broad prospects. We sincerely invite you to cooperate with us, please contact us to provide frog venom expertise, service and support.

Reference

• Fusco L S, et al. Biochemical characterization and cytotoxic effect of the skin secretion from the red-spotted Argentina frog Argenteohyla siemersi (Anura: Hylidae). Journal of Venomous Animals and Toxins including Tropical Diseases, 2020, 26(10).