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Functional Activity Analysis of Venom Peptide/Protein

The toxicological effects of venom peptide/protein are very extensive. Taking the human body as an example, their functions cover almost every physiological system of the human body. Among them, it is closely related to key parts of the blood system, immune system, and nervous system. Many peptides and proteins in animal toxins can act on various factors in the blood system, a variety of cell membrane receptors and ion channels, and the complement system. In addition, many venom peptides/proteins have a very strong killing effect on microorganisms, especially bacteria. Creative Proteomics can help you complete a series of venom peptide and protein functional activity detection services, and help you obtain high-quality results for follow-up research.

VSnake venom mainly contain
Neurotoxicity, cytotoxicity, protease inhibitors, phospholipase A2, disintegrin, natriuretic peptide, angiogenesis factor, bradykinin enhancing peptide, nerve growth factor, immune rejection inhibitor CVF, lectin, C-type Lectins, serine proteases, metalloproteinases, cysteine-rich snake venom protein (CRVP), other enzymes (mainly including amino acid oxidase, hyaluronidase, 5'nucleosidase, cholinesterase, etc.).
VAmphibian skin secretions mainly contain Biogenic amines, bufogenin, alkaloids, peptides and proteins. (Antibacterial peptides, serine protease inhibitors, bradykinin, angiotensin-like peptides, bombesin, cerulein, frogmorphin, chemotactic frog peptides, Tryptophillin peptides, Xenopus peptides, Xenopus calmodulin genes Related peptides, proteases, etc.)
Arthropod venom glands mainly contain Allergens, antiplatelet aggregation factors, immunosuppressive peptides, thrombin inhibitors, anticoagulant silk amino acid proteases, fibrinogen hydrolase, three families of antimicrobial peptides, hyaluronidase, vasodilatory factors, antioxidants Metallothionein, ion channel activator/antagonist, etc.

Antibacterial Activity Detection

Determine the effect of purified venom peptides or proteins on inhibiting bacterial growth in vitro. Through this experiment, the minimum inhibitory concentration can be determined to evaluate the antibacterial performance of the extract. The main methods are the diffusion method for qualitative determination and the dilution method for quantitative determination.

Hemolytic Activity Detection

The hemolysis test is to detect the red blood cell rupture and lysis of the red blood cells in the human body. Mainly used for the diagnosis of hemolytic anemia. Hemolysis refers to the rupture and dissolution of red blood cells. Many physical and chemical factors can cause hemolysis.

Measurement of PLA2 Activity Detection

PLA2 enzymes are commonly found in mammalian tissues as well as arachnid, insect, and snake venom. Venom from both snakes and insects is largely composed of melittin, which is a stimulant of PLA2. Due to the increased presence and activity of PLA2 resulting from a snake or insect bite, arachidonic acid is released from the phospholipid membrane disproportionately. As a result, inflammation and pain occur at the site. Therefore, the detection of PLA2 enzyme activity is an important part of understanding the venom peptide/protein.

More Detections

The composition of venom peptide/protein is very complex and its effects are diverse. Creative Proteomics provides various basic venom peptide/protein research techniques to help your in-depth study of the various characteristics.

  • Antioxidant Activity Assays
  • Edema-forming Activity Detection
  • Procoagulant Activity Detection
  • Immunochemical Characterization Detection

Creative Proteomics can help you complete a series of functional activities detection services, and help you obtain high-quality results for follow-up research.

Please contact us for more information.

For research use only. Not intended for any clinical use.
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