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Venom Target Protein Analysis

Animal toxins range from small organic molecules to large proteins over 100 kDa. Targeted proteomics is a science that is developed on the basis of proteomics to study specific target proteins. As a method of detecting target proteins in protein quantification based on mass spectrometry, targeted proteomics analysis technology has the characteristics of high sensitivity, high quantitative accuracy and repeatability. It can be used for signal transduction pathway detection, protein post-translational modification research and marker research, etc. MRM and PRM technologies are two commonly used technologies in targeted proteomics. We provide MRM and PRM targeted proteomics analysis services.

Targeted Proteomics in Venom

MRM (multiple reaction monitoring), also known as SRM (selected reaction monitoring). This technology uses triple quadrupole mass spectrometry, which uses the high selectivity of the quadrupole to sort the parent ions and product ions in sequence, collect signals that meet the target ion rules, remove other signal interference, and quantify the target protein.

PRM (parallel reaction monitoring) is a technology derived from MRM, which combines the high selectivity of the quadrupole with the high resolution and high precision of Orbitrap. It can independently identify the secondary atlas and has better detection sensitivity and anti-interference ability. The targeted proteomics technology process is divided into three steps:

Method Development Establishment

The first step of targeted proteomics analysis is to develop a suitable analysis process for the target sample and use the HRM method to build the corresponding spectrum database. For the protein of interest, a series of detection methods (also called transition or exclusion lists) need to be established and optimized. Foresee

The analysis needs to obtain the characteristic information of the peptide peak, such as fragment ion intensity and retention time (iRT), these results are the reference for the formal experimental data collection.

Data Collection

To collect data, triple quadrupole mass spectrometry (MRM) or high-resolution Orbitrap mass spectrometry (PRM) is used. Both of these methods require a front quadrupole to select target ions from the pre-experiment, and then introduce the ions into the collision cell. The MRM method only detects preselected ions in the third quadrupole, while PRM uses its high-resolution detector to detect all fragment ions, thereby quantifying more protein in a single analysis.

Data Analysis

Data analysis of large-scale samples has always been a shortcoming of MRM and PRM, requiring researchers to input a large number of parameters for optimization analysis. The SpectroDive developed by Biognosys overcomes these challenges. It has the most intelligent peak extraction algorithm available, and can realize the automated analysis of large-scale samples.

Creative Proteomics' targeted proteomics analysis platform is the latest and most advanced analysis tool in large-scale sample and large-scale protein verification research. Our services include experimental method establishment, formal sample testing, data analysis and other parts. We will provide customized services according to your needs and samples.

Please contact us for more information.

For research use only. Not intended for any clinical use.
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