Integrated Analysis of Lipidomics and Proteomics

Introduction

The integrated analysis of venom lipidomics and proteomics refers to the normalization and statistical analysis of batch data from the venom proteomics and lipidomics, and the establishment of different levels of intermolecular data relationships. At the same time, it combines venom functional analysis, metabolic pathway enrichment, molecular interaction and other biological function analysis to systematically and comprehensively analyze the function and regulation mechanism of biomolecules, and finally realize a comprehensive understanding of the general trend and direction of biological changes. The integrated analysis of lipidomics and proteomics on toxic species or the venom secreted by them can provide new research perspectives for venom diversity, evolutionary biology and pharmacology.

What Creative Proteomics Offers

Creative Proteomics’s venom lipidomics service adopts advanced tandem mass spectrometer and analysis software, can provide customized detection solutions, fast and accurate analysis services, and help customers with lipid metabolomics detection. In addition, we can also provide a variety of venom proteomics quantitative and qualitative services. Combined with the integration of customizable bioinformatics analysis methods, Creative Proteomics provides you with one-stop venom lipidomics and proteomics integrated analysis services from experimental design, sample testing, and data analysis to meet a variety of testing needs.

Method Flow

Fig 1. Lipidomics and proteomics integrated analysis process - Creative Proteomics

Integrate Analysis Content

1. Multivariate statistical integration analysis of venom proteome and lipidome, including O2PLS association analysis, and selection of OPLS related variables.
2. When the number of biological repeats is ≥15, weighted ene co-expression network analysis (WGCNA) can be performed.
3. Correlation analysis between venom proteome and lipidome.
4. Correlation coefficient matrix heat map, correlation analysis hierarchical clustering heat map, correlation coefficient control network analysis.
5. Comparative analysis of PCA between venom proteome and lipid group (limited to ≥3 groups).

Solved Problem

Through the integrated analysis of venom lipidomics and proteomics, the following problems can be solved:

• Realize the full spectrum analysis of venom proteins and lipids by analyzing the expression levels of different levels.
• At the same time, it is possible to explore biological issues from both the cause and effect directions, and the mutual verification effect is more obvious.
• Explain the association mechanism between molecular regulation and phenotype, and systematically and comprehensively analyze the function and regulation mechanism of venom.
• Remove the false from the massive data, screen out the key lipids and proteins for subsequent in-depth experimental analysis and application.

Project Report

In the result report, Creative Proteomics will provide you with a detailed technical report, including:

1. Experimental procedure (LC-MS/MS).
2. Related experimental parameters.
3. Mass spectrum picture.
4. The raw data of sequencing, including lipidomics data and proteomics data.
5. Analysis software and parameters used.
6. Graph of results of integrated analysis of lipidomics and proteomics data.
7. Chart information interpretation.

How to Order?

Creative Proteomics provides customers with professional integrated analysis of lipidomics and protemics data analysis services, aiming to help you understand the venom from the lipid and protein level. We have powerful bioinformatics analysis experts, and can provide you with various types of analysis services according to your needs. For high-throughput sequencing and integrated analysis of lipidomics and protemics bioinformatics data analysis, if you have any questions, please feel free to contact us.