Sequence Confirmation Analysis Service
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The process of antibody drug development and process establishment requires confirmation of the full-length sequence of a protein. Creative Proteomics provides advanced protein sequence technology services with our high-resolution mass spectrometry technology platform, which enables 100% coverage of the target protein sequence. This analysis service helps to confirm the correct amino acid sequence of antibody drugs, ensuring their safety and efficacy.
Coverage and Importance of Sequence Confirmation
Sequence confirmation is an essential part of antibody drug development and quality control and is critical to ensuring the intended therapeutic properties of antibody drugs and the overall safety of the drug. At Creative Proteomics, we offer comprehensive sequence confirmation services for antibody drugs to ensure 100% coverage. Coverage is the ratio of sequences sequenced to the full sequence size. It reflects how well our sequencing results match the target sequence. If the coverage is less than 100%, we will review the protein data and sequences. Based on the actual results, we determine whether the protocol needs to be optimized to reacquire the missing positions of the peptide, or if the protein sample itself is mutated and requires de novo sequencing analysis. Our experienced team of experts is dedicated to complete and detailed analysis of the amino acid sequences of the samples to ensure accuracy and reliability.
Sequence Confirmation Methods at Creative Proteomics
Our sequence confirmation method combines state-of-the-art technology and industry best practices to deliver high-quality results. We employ a variety of techniques, including high-resolution mass spectrometry, peptide mapping, and gene sequencing, to comprehensively analyze the protein structure and confirm the sequence with utmost accuracy.
Mass spectrometry is a common technique for sequence confirmation and allows accurate and sensitive detection of any amino acid modification or change.
Sequencing techniques such as Edman degradation can provide detailed sequence information and can help identify any post-translational modifications that may have occurred in the antibody drug. In addition, DNA sequencing technologies provide an additional level of confirmation and can detect any potential errors or mutations in the gene sequence.
Confirmatory methods like peptide mapping, N-terminal sequencing, and liquid chromatography can be employed to cross-validate the results obtained from mass spectrometry or gene sequencing techniques.
Sequence Confirmation Service Flow
The proteins are cleaved using a combination of multiple proteases to improve the cleavage efficiency in order to obtain the sequence information of each amino acid of the protein.
Analysis of samples using high-resolution liquid mass spectrometry.
The obtained information on the mass to nucleus ratio was compared with the database established by theoretical sequences.
The peptides identified by each digestion method were spliced to obtain information on whether the protein was correctly expressed intact.
Creative Proteomics combines our expertise with advanced equipment to perform comprehensive full sequence validation analysis of antibody proteins to ensure the safety and efficacy of antibody drugs for your research and quality control demands. Contact us to learn more about our sequence confirmation analysis service for antibody drugs. We will be happy to assist you.
