In the Ag-Ab affinity service, different concentrations of the Ag and Ab are tested in a series of experiments. By measuring the level of binding or the rate of binding between the two molecules, the affinity is determined. A higher affinity constant suggests a stronger relationship between the Ag and Ab, indicating a more specific and potent immune response. Creative Proteomics provides professional Ag-Ab affinity services to help researchers and clients gain insight into the strength of the immune response so that they can effectively evaluate the efficacy of antibody drugs.
Introduction of Ag-Ab
Antigen-Antibody (Ag-Ab) Interaction refers to the biochemical reaction that occurs between antibodies and specific antigens when they come into close proximity, typically within a distance of several nanometers. This interaction plays a critical role in the immune response against foreign substances and pathogens in the body. The interaction between antigens and antibodies forms the basis of various diagnostic and research applications. Ag-Ab affinity assay is a powerful technique used to measure the strength of the binding interaction between antigens and antibodies. This quantitative assessment of binding affinity provides valuable insights into the specificity and effectiveness of the immune response.
Fig. 1 Antigen-antibody (Ag-Ab) interaction. (Mayer, G., 2010)
Our Ag-Ab Affinity Assay Services
The Ag-Ab affinity assay is designed to evaluate the binding affinity between antigens and antibodies using advanced bioanalytical techniques. Through this assay, the affinity constant, also known as the dissociation constant (Kd), can be determined to quantify the strength of the interaction between the target antigen and the specific antibody.
At our state-of-the-art laboratory, we employ cutting-edge technologies and follow rigorous protocols to ensure accurate and precise measurements of Ag-Ab binding affinity. Our experienced team of scientists and technicians are well-versed in various assay formats, including enzyme-linked immunosorbent assays (ELISA), surface plasmon resonance (SPR), and immunofluorescence, among others. This allows us to cater to a wide range of research needs and deliver reliable results for both small-scale projects and large-scale studies.
Service Highlights
- Comprehensive assay design
Our dedicated team of experts will collaborate with you to understand your specific research objectives and design a customized assay protocol tailored to your requirements.
- Optimal assay performance
We take into account various factors like the nature of the antigen and antibody, sample size, and downstream applications to ensure optimal assay performance.
We only utilize high-quality reagents, including purified antibodies and validated antigens, to maintain assay integrity and accuracy.
Creative Proteomics combines our expertise with state-of-the-art testing platforms to help clients around the world realize the acceleration of their antibody drug development process. Contact us to learn more about our Ag-Ab affinity assay service of antibody drugs. We will be happy to serve you.
Reference
- Mayer, G. Immunoglobulins antigen-antibody reactions and selected tests. Microbiology and Immunology. 2010.
