The detection of apoptosis is a tool for antibody drug discovery and an experimental means to validate drug efficacy in vitro, which helps to elucidate the anti-tumor mechanism of antibody drugs. Creative Proteomics' s apoptosis assay service offers a robust, companionate solution allowing pharmaceutical companies to craft superior antibody drugs, by providing insight into the bioactivity of these treatments.
Impact of Apoptosis Assay on Biological Activity
Apoptosis, or programmed cell death, is a crucial physiological process that plays an integral role in determining the efficacy of antibody drugs. The interaction between these drugs and target cells often triggers the process of apoptosis, consequently impacting the treatment response. Understanding the ability of these biotherapeutics to instigate cell death is, therefore, an essential parameter when it comes to designing effective antibodies.
Fig. 1 The evaluation of ADC-induced tumor apoptosis. (Tsuboi, S. et al., 2022)
Our Apoptosis Assay Service
Our team of experts utilizes state-of-the-art techniques and technologies to conduct apoptosis assays. We offer in vitro comprehensive assay to evaluate the efficacy and safety of antibody drugs in inducing apoptosis. Our goal is to provide reliable and accurate data to guide the development of antibody drugs and facilitate the decision-making process for drug developers.
Our apoptosis assay service includes the following key components:
We work closely with our clients to tailor the apoptosis assay according to their specific requirements. Our team of scientists will assess the target cells and determine the most suitable assay conditions, including cell type, concentration of antibody drugs, and incubation time.
- Cell viability assessment
We employ various techniques to measure cell viability and assess the cytotoxic effect of antibody drugs. This includes using flow cytometry-based assays, such as Annexin V staining, to quantify apoptotic cells and assess cell death.
- Caspase activity measurement
Caspases are key enzymes involved in apoptosis. We offer caspase activity assays, such as caspase 3/7 activity assays, to evaluate the activation of caspases in response to antibody drugs. This provides valuable insights into the mechanism of action of the antibody drugs.
- Data analysis and interpretation
We provide comprehensive data analysis and interpretation services.
Our Apoptosis Assay Service Features
- We utilize relevant cell lines that are known to express specific apoptotic markers or are derived from disease models.
- We include positive and negative controls to ensure the validity and accuracy of the assay.
- We employ fluorescence-based detection reagents to measure apoptotic cell populations accurately.
- We generate detailed reports that include the percentage of apoptotic cells and statistical analysis of the results.
Creative Proteomics has extensive project experience in characterizing the bioactivity of antibody drugs. Our apoptosis assay service for antibody drugs is designed to evaluate the pro-apoptotic activity of antibodies in order to identify potential candidates for drug development. Contact us to learn more about our services. We will be happy to serve you.
Reference
- Tsuboi, S.; et al. In Vitro and In Vivo Fluorescence Imaging of Antibody-Drug Conjugate-Induced Tumor Apoptosis Using Annexin V-EGFP Conjugated Quantum Dots. ACS omega. 2022, 7(2): 2105-2113.
