FcγRs affinity assay is designed to measure the binding affinity between FcγRs and antibody drugs. Creative Proteomics offers comprehensive FcγRs binding assay utilizing our advanced technology platform. This assay provides important information on the binding of antibody drugs to FcγRs and trigger immune effector functions. By evaluating the affinity of an antibody drug for different FcγR subtypes, researchers can gain insights into the drug's potential efficacy and safety profile.
Introduce of FcγRs
FcγRs (Fc-gamma receptors) are a family of cell surface receptors that play a crucial role in the immune system's response to various pathogens and diseases. These receptors are responsible for binding to the Fc region of immunoglobulins (antibodies) and mediating several immune functions, such as phagocytosis and antibody-dependent cell-mediated cytotoxicity. Understanding the affinity of FcγRs is essential in the context of antibody-based therapeutics and immunotherapy. The affinity determines the strength of the interaction between FcγRs and antibodies, which directly affects the efficiency of immune responses. Higher-affinity FcγRs can bind to antibodies more tightly, leading to enhanced immune cell activation and effector functions. Therefore, in antibody-based therapeutics, such as monoclonal antibodies (mAbs), understanding and selecting the appropriate FcγR binding affinity is critical. By choosing FcγRs with high affinity for a specific antibody, therapeutic efficacy can be optimized.
Fig. 1 The family of human FCΓRS. (Vogelpoel, L. T. et al., 2015)
Our FcγRs Affinity Assay Services
Our FcγRs affinity assay service for antibody drugs is a specialized offering designed to analyze the binding affinity between antibody drugs and Fcγ receptors (FcγRs). Our highly experienced team utilizes state-of-the-art technologies and methodologies to conduct rigorous affinity assays, including but not limited to the following:
- Surface plasmon resonance (SPR)
Surface plasmon resonance is a label-free, real-time, and high-throughput technique used for studying molecular interactions, including protein-protein interactions. Creative Proteomics utilizes surface plasmon resonance for Fc receptor binding assays. Our SPR-based affinity testing service for FcγRs enables researchers to accurately measure the binding affinity and kinetics between FcγRs and antibodies. This information helps in the identification and selection of antibodies for various therapeutic applications.
Flow cytometry allows for the quantitative measurement of antibody drug affinity for FcγRs. In this assay, we incubate cell populations expressing FcγRs with different concentrations of antibody drug. The cells are then stained with a fluorescent tag specific for the antibody drug, allowing for the detection and quantification of bound antibodies. By analyzing the fluorescence intensity of the stained cells, we can help our clients determine the affinity of the antibody for FcγRs.
- Enzyme-linked immunosorbent assays (ELISA)
The enzyme-linked immunosorbent assay (ELISA) is a commonly used method for the determination of antibody-antigen interactions. At Creative Proteomics, we immobilize FcγR proteins on solid surfaces such as microtiter plates. After immobilizing the FcγR protein, different concentrations of antibody drugs are added to the plate to bind to the FcγR. After washing away unbound antibody, a secondary antibody conjugated to an enzyme, such as horseradish peroxidase (HRP) or alkaline phosphatase (AP), is added. This secondary antibody recognizes and binds to the Fc region of the antibody drug, enabling detection of the bound antibody. By measuring the absorbance or fluorescence of the product, we can help our clients determine the binding affinity of the antibody drug to the FcγR. The affinity is usually expressed as a dissociation constant (Kd), which represents the concentration of the antibody drug to the FcγR.
Service Highlights
- Specialized testing platform with over ten years of experience.
- Standardized and customized testing services to meet diverse testing demands.
- Evaluation of the binding affinity against different isoforms and subtypes of Fc gamma receptors, such as FcγRI, FcγRIIa, FcγRIIb, FcγRIIIa, and FcγRIIIb. Comprehensive description of antibody interactions with different receptors.
Creative Proteomics combines our expertise with state-of-the-art testing platforms to help clients around the world realize the acceleration of their antibody drug development process. Contact us to learn more about our FcγRs affinity assay service of antibody drugs. We will be happy to serve you.
Reference
- Vogelpoel, L. T.; et al. Control of cytokine production by human fc gamma receptors: implications for pathogen defense and autoimmunity. Front Immunol. 2015 Feb 24; 6: 79.
