Intact Molecular Weight

Determining the intact molecular weight of a specific antibody drug is critical to ensure its efficacy and safety. The intact molecular weight of an antibody drug varies depending on the specific drug. Creative Proteomics uses our advanced technology for intact molecular weight determination of antibody drugs to help you obtain relevant data. We have established accurate and reliable molecular weight determination analytical methods to characterize the true size of antibody drugs and to guide the R&D design, production process optimization and quality control of antibody drug products.

What is Intact Molecular Weight of Antibody Drugs?

Intact molecular weight determination is an essential characterization component in antibody drug development and production, and the most direct results can be obtained at the intact protein level.

Intact molecular weight determination of an antibody drug is a crucial analytical process that involves the measurement and calculation of the total mass of the molecule. Accurate mass measurements help determine whether the expected post-translational modifications (PTM) are expressed as the correct protein sequence, and they also provide the relative abundance of different antibody proteins or PTMs in the same sample. It starts with the determination of the mass of the heavy and light chains comprising the antibody protein. This is achieved by techniques such as mass spectrometry, which ionizes and accurately measures the mass-to-charge ratio of the molecules. Additionally, the determination includes the measurement of post-translational modifications, such as glycosylation or disulfide bonds, which can further affect the molecular weight.

Protein integrity mass number analysis is an important component of characterizing antibody drugs. Direct mass measurements allow the assignment of chemical modifications that contribute to product heterogeneity. Measuring full and partial molecular species in antibody drugs to characterize protein amino acid sequences, identify structural domain-specific modifications, analyze glycoforms and assess possible changes under in vivo conditions are effective methods for identifying protein features that may be susceptible to modifications that may be key attributes for stability, efficacy and safety.

The content of this test includes:

• Complete molecular weight size of the antibody drug.
• Presence of major glycoforms.
• Presence of free light chain and/or heavy chain plus semi-antibodies.
• Presence of non-glycosylated species.

Intact Molecular Weight Determination Methods and Procedures

The techniques we offer for the determination of the intact molecular weight of antibody drugs include a combination of mass spectrometry (MS) and liquid chromatography (LC). These advanced technology combinations can help customers obtain accurate information on the molecular weight of antibody drugs. Our detailed testing procedures include the following:

• Sample preparation: Antibody drug samples are carefully purified and processed to remove human deterioration of impurities that may interfere with the analysis.
• Sample analysis: The purified samples are introduced into a liquid chromatography system and separated according to their size and weight. This step ensures that only intact antibody molecules are analyzed.
• The separated sample is introduced into the mass spectrometer, which determines the mass point he must be and generates the required molecular weight information.
• Data analysis: The detected data is analyzed and interpreted to provide a complete analytical report of the antibody drug molecular weight study.

Advantages of Our Intact Molecular Weight Determination

• Experienced testing team.
• Fast and accurate, enabling relatively quantitative LC/MS techniques.
• Advanced data analysis software that provides an intuitive form of displaying test reports.

Creative Proteomics is committed to providing comprehensive, convenient, and professional antibody drug characterization services with rapid delivery using our well-established technology service platform. Contact us to learn more about intact molecular weight determination service for antibody drugs. We will be happy to assist you.