Neutralizing/Blocking Antibody Validation
Online Inquiry
Neutralizing or blocking validation is a crucial step in the confirmation of antibody functionality pertaining to specific biological processes. At Creative Proteomics, we offer comprehensive neutralizing/blocking antibody validation services designed to test and verify the functionality of antibodies and facilitate the development of antibody drugs. Our team of experienced scientists and technicians are available to conduct in-depth analyses and provide accurate results that will inform your antibody drug development efforts.
Introduction to Neutralizing or Blocking Antibodies
Neutralization or blockade is a good tool for detecting the specificity of antibody binding to the intended target. Neutralizing antibodies are a class of antibodies that possess antiviral activity that recognizes viral surface proteins, prohibits the virus from binding to the cell and blocks its function. Blocking antibodies are those that are able to bind directly to proteins, thereby affecting their function. These two types of antibodies are widely used and play an important role in the development of antibody drugs.
Our Neutralizing/Blocking Antibody Validation Service
At Creative Proteomics, we aim to provide our clients with the best practices in research and development and one such service is the neutralizing/blocking antibody validation service through cell-based assays. The primary objective of this service is to assess the effectiveness of specific neutralizing antibodies in blocking or neutralizing the biological activity of target proteins in cellular systems.
Our accurate, efficient validation process consists of the following:
- Selection of the target cells and molecules
Our validation process begins with the careful selection of the target cells and molecules. Through this selection, we aim to create an accurate representation of the cell environment in which the antibody will be used.
- Cell-based quantitative assay
We will establish a quantitative cell-based assay to measure the function of the target protein according to the customer's demands. Our experienced scientists will provide an assay that is continuously optimized to ensure its sensitivity, selectivity and reliability.
We perform the assay in the presence and absence of the neutralizing/blocking antibody to assess if it can indeed downregulate or block the function of the target protein. we place a strong emphasis on delivering quantitative and reproducible results. As a result, we include several replicates and control groups in our assay to account for variability and provide reliable information on the antibody's neutralizing/blocking effects.
Our professional data analysis team provides you with comprehensive data analysis to determine the inhibition rate, calculate IC50 or EC50 values, and construct dose-response curves. These data render a clear understanding of the antibody's bioactivity.
Neutralizing/Blocking Antibody Validation Service Features
- Professional testing platform with over 10 years of project experience.
- Efficient one-stop services for customers.
- High-quality assurance and short turnaround time.
Creative Proteomics adheres to the highest standard of protocols throughout all steps of the process to provide trustworthy and actionable reports. Contact us to learn more about our services. We will be happy to serve you.
