Phosphorylation modification characterization services play a crucial role in the development and optimization of antibody drugs. Creative Proteomics utilizes our powerful mass spectrometry tools in conjunction with comprehensive data analysis to provide detailed information on the sites of phosphorylation of antibody drugs.
Importance of Phosphorylation Analysis
Phosphorylation, the addition of a phosphate group to a protein or antibody, is a common post-translational modification that can significantly impact the function, stability, and efficacy of the drug. Phosphorylation events exert control over many cellular processes, including signal transduction, protein-protein interactions, and enzyme activity. Understanding the phosphorylation status of an antibody drug is essential for comprehending its mechanism of action, as well as for predicting potential efficacy and safety issues. This is particularly relevant in the context of antibody-based therapeutics, as antibodies interact with a wide variety of molecular targets and signaling pathways.
Our Phosphorylation Modification Characterization Services
Using advanced techniques and state-of-the-art equipment, we can accurately determine the phosphorylation sites within your antibodies, providing valuable insights into their functional and regulatory properties. Our services include:
- Phosphorylation site mapping.
- Quantitative phosphorylation analysis.
- Assessing the effect of phosphorylation on characteristics such as binding affinity, potency and efficacy of antibody drugs.
Phosphorylation Characterization Techniques at Creative Proteomics
At Creative Proteomics, we have a wide range of analytical techniques with advanced physics. Among them, mass spectrometry (MS) is one of our commonly used analytical tools as it provides detailed information on the site and stoichiometry of phosphorylation events. Combined with our mass spectrometry-based high-throughput phosphorylation proteomics platform, phosphorylation events can be analyzed across the entire proteome, providing a global view of antibody drug phosphorylation.
In addition to MS-based techniques, other methods such as Western blotting and enzyme-linked immunosorbent assay (ELISA) can be employed to assess the phosphorylation status of specific residues or domains within the antibody drug. These techniques can be used to verify the results obtained from MS-based approaches and provide further validation.
Phosphorylation Characterization Services Process
Our experienced scientists can provide customized antibody drug phosphorylation characterization services to meet specific customer needs. Our one-stop service begins with sample preparation, purification of the antibody drug and enzymatic digestion to release the phosphorylated peptide. Next, the phosphorylated peptide is enriched using an affinity agent and the phosphorylation site in the antibody drug is detected and identified by mass spectrometry-based techniques. Once the phosphorylation sites are identified, the degree of phosphorylation of each site is quantified by our customized labeling strategy, ensuring comprehensive phosphorylation information for our clients.
Features of Our Phosphorylation Modification Characterization Service
- Customized and flexible experimental design.
- Advanced analytical techniques and high-throughput mass spectrometry analysis.
- Detailed reports including quantitative analysis of phosphorylation sites, identification of novel phosphorylation events, and determination of phosphorylation motifs.
Creative Proteomics provides our clients with authentic, reliable and timely characterization analysis with our advanced technology platform. Contact us to learn more about our phosphorylation modification characterization services of antibody drugs. We will be happy to assist you.
