In Vitro Drug Metabolism Assays
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In vitro metabolism assay services for antibody drugs are used to assess the metabolic stability and biotransformation of antibody-based therapeutics. Creative Proteomics offers a wide range of in vitro assays designed to comprehensively assess the metabolism of antibody drugs. These assays can provide valuable information on the metabolic fate of antibody drugs, including the identification of major metabolic pathways, the formation of toxic or inactive metabolites, and the potential for drug-drug interactions.
Introduction of In Vitro Metabolism Assay Services for Antibody Drugs
In vitro metabolism assay services are designed to evaluate how the drug interacts with metabolic enzymes, such as cytochrome P450 enzymes, and determine its potential for drug-drug interactions and clearance in the body. In vitro metabolism assays for antibody drugs typically involve incubating the drug with human or animal liver microsomes, which contain a rich mixture of metabolic enzymes. The reactions are performed under controlled conditions to mimic the physiological environment in which the drug would be metabolized in vivo. Researchers then analyze the resulting metabolites using various analytical techniques, such as liquid chromatography-mass spectrometry (LC-MS), to identify and characterize the metabolic products.
In Vitro Metabolism Assay Services for Antibody Drugs at Creative Proteomics
Our experienced scientists utilize state-of-the-art equipment and well-established protocols to perform in vitro metabolism assays. Our capabilities include:
We employ state-of-the-art techniques to identify and quantify metabolites generated during the biotransformation of antibody drugs. These techniques include liquid chromatography-mass spectrometry (LC-MS), nuclear magnetic resonance (NMR) spectroscopy, high-resolution mass spectrometry (HRMS) and enzyme-linked immunosorbent assay (ELISA). This enables us to gain insights into the metabolic pathways and potential toxicity of these compounds, supporting drug development and regulatory submissions.
By measuring parameters such as Km and Vmax, we can evaluate the enzymatic activity and predict potential drug-drug interactions or metabolic liabilities.
By utilizing a comprehensive panel of recombinant enzymes, we can identify and quantify the contribution of different enzymes involved in drug metabolism, enabling informed decision-making during drug development.
- Drug-drug interaction studies
Through inhibition and induction assays, we evaluate the impact of competing drugs on the metabolism of antibody therapeutics, providing valuable insights into potential drug interactions.
Services Features of Our In Vitro Metabolism Assay Services
- High-throughput screening
Our in vitro metabolism assay service allows for rapid and automated screening of a large number of antibody drug candidates.
- Comprehensive metabolic profiling
Our service provides a comprehensive metabolic profiling of antibody drugs, allowing researchers to understand how these drugs are metabolized in different biological matrices.
- Customizable assay formats
Our experienced team can design customized assays, such as liver microsomes, hepatocyte incubation, or cytosolic fractions, based on the specific requirements of the antibody drugs being tested.
Creative Proteomics is pleased to introduce a state-of-the-art in vitro antibody drug metabolism assay service to help pharmaceutical companies and researchers streamline the drug development process. For more information or to request our services, please feel free to contact us.
