Molecular Wieght Determination
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Antibody drug molecular weight testing is a key quality control process in biopharmaceutical development and manufacturing. As a professional antibody drug characterization service provider, Creative Proteomics' advanced antibody molecular weight analysis platform provides ultra-high resolution, high sensitivity, and high precision assays to help clients fully characterize the molecular weight of antibody drugs.
Introduction of Antibody Drug Molecular Weight Testing Service
Antibodies are large and complex molecules with high molecular weights. Molecular weight testing of antibodies includes the following:
During the test, the molecular weight can be directly detected by high-resolution MALDI-TOF or ESI-MS. The detection of the post-deglycosylation molecular weight allows for a preliminary characterization of the antibody drug and will be used as a routine release method for the drug. The analysis of antibody drugs before deglycosylation can provide information on the type of glycosylation and the distribution of glycosylation levels of antibody drugs, which is very important for the rapid understanding of the relationship between the production process and drug quality.
Molecular Weight Detection Method
Molecular weight is divided into intact molecular weight and reduced molecular weight to characterize the antibody molecule as a whole and to confirm whether there are fragment deletions or mismatches in the antibody molecule. For double antibodies or other multiple antibodies, the difference in molecular weight between the target molecule and the mismatched molecule (e.g. homodimer) is small, as small as a dozen Da, and the difference in physicochemical properties is small, making it impossible to distinguish the mismatch of double antibodies by conventional chromatographic methods. Creative Proteomics has a variety of advanced detection techniques to accurately characterize the molecular weight of antibody drugs.
Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) can be used to analyze the large molecular weight and structure of different forms of antibodies, such as intact antibodies or their subunits, and to identify potential modifications, such as glycosylation or oxidation. This method is performed by shooting a laser at a target, which ionizes and splits the sample into charged molecules that are subsequently separated and detected based on their mass-to-charge ratios. The resulting mass spectrum represents the mass-to-charge ratio of ions in the sample and can be used to determine the molecular weight and confirm the identity of the antibody drug. The peak intensity and distribution of ions can also reveal information about the purity and homogeneity of the antibody sample. MALDI-TOF-MS is a common technique for molecular weight characterization of antibody drugs in our laboratory because of its rapidity, ease of use, high sensitivity, and wide range of detectable masses.
Electrospray ionization mass spectrometry (ESI-MS) provides a highly sensitive and accurate analytical method to determine the molecular weight of antibody drugs with high precision. The technique is based on the ionization of analytes in a charged droplet spray and then into a mass spectrometer, where mass analysis is performed. In the case of antibody drugs, ESI-MS can provide information on the molecular weight of the entire antibody molecule as well as the molecular weights of its various subunits. In addition, any post-translational modifications, such as glycosylation or phosphorylation, can be detected.
Molecular Weight Testing Process
Service Advantages
- Project experienced testing team.
- Advanced mass spectrometry detection and analysis technology.
- Ultra-high resolution, high sensitivity and high-quality precision assays.
Creative Proteomics utilizes an advanced characterization technology platform to provide authentic, reliable, and timely characterization services. Contact us to learn more about our molecular weight characterization services for antibodies and we will be happy to assist you.
