ADCP Activity Test

ADCP activity plays a crucial role in the efficacy of antibody-based therapeutics, such as antibody-drug conjugates (ADCs). Monitoring and quantifying ADCP activity can provide valuable insights into the effectiveness of these drugs and aid in their development. At Creative Proteomics, we specialize in providing state-of-the-art ADCP activity assay service. Our team of highly skilled scientists and experts offer a comprehensive range of solutions to assess the potency and efficacy of antibody drugs.

Why is ADCP Activity Testing Necessary?

Antibody-dependent cell-mediated phagocytosis (ADCP) is a vital mechanism by which the immune system eliminates cells, pathogens or other foreign materials. This process involves the recognition and engulfment of target cells by phagocytes, like macrophages or neutrophils, via the binding of antibodies to their surface antigens. ADCP activity testing is essential for characterizing the efficacy of antibody drugs. It helps assess the phagocytic potential of antibodies, predict therapeutic response, and guide drug development decisions.

Fig. 1 Antibody-dependent cellular phagocytosis (ADCP). (Van, Erp. E. A. et al., 2019)

Our ADCP Testing Services

At Creative Proteomics, we have established standard processes for accurately measuring ADCP activity. Our advanced assay help customers accurately quantify the ADCP activity of antibody therapeutics. Our ADCP activity test service includes the following key features:

• Cell-based assay: We utilize a validated cell-based assay to measure the ability of the antibody drug to promote phagocytosis of target cells by macrophages. We use a fluorescent readout to quantify the phagocytosis events and calculate the ADCP activity.
• Assessment of multiple target cells: We offer the flexibility to use various target cells, including cancer cell lines or cells expressing specific antigens, based on the client's needs. Our experts can assist in selecting the most appropriate target cells for the specific antibody drug being tested.
• Evaluation of different effector cells: In addition to macrophages, we can also assess the ADCP activity of antibody drugs using other effector cells, such as dendritic cells or neutrophils, if required.
• Standardized protocols and controls: We follow standardized protocols and include appropriate positive and negative controls to ensure the reliability and reproducibility of the results.
• Data analysis and interpretation: Upon completion of the ADCP Activity Test, we provide a comprehensive report summarizing the experimental details, results, and data analysis. Our team of experts can also assist with data interpretation and provide scientific insights.

ADCP Testing Service Features

• Our test provides a comprehensive assessment of ADCP activity.
• Our test utilizes state-of-the-art techniques and technologies, ensuring high sensitivity to detect even subtle changes in ADCP activity.
• Our testing protocols are highly customizable, allowing you to tailor them to your specific demands.

Creative Proteomics offers ADCP activity testing services that help researchers and pharmaceutical companies gain valuable information about the effectiveness of their antibody drugs in triggering immune-mediated killing or clearance of target cells. Contact us to learn more about how our antibody drug ADCP activity testing service can contribute to the success of your drug development efforts.

Reference

1. Van, Erp. E. A.; et al. Fc-mediated antibody effector functions during respiratory syncytial virus infection and disease. Frontiers in immunology. 2019, 10: 548.