Deglycosylated Molecular Weight
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Changes in the glycosylation pattern of antibody drugs may significantly alter their intrinsic properties and stability. Creative Proteomics' deglycosylated molecular weight determination service provides essential information on the glycosylation pattern and molecular weight of antibody drugs, facilitating a comprehensive analytical characterization of the molecular weight of antibody drugs.
Introduction to Deglycosylated Molecular Weight Determination
Antibody glycosylation is a common post-translational modification that plays a key role in antibody stability, function, and immunogenicity. Therefore, deglycosylated molecular weight (DMW) assays can provide insights into the glycosylation patterns of antibody drugs and their impact on overall molecular weight. Our scientists provide methods for the efficient preparation of fully deglycosylated antibodies and the accurate determination of their molecular weight, providing valuable information about the overall structure and function of the drug. With our accurate assay services, we can help our customers ensure the consistency, efficacy and safety of their products and avoid the effects of potential adverse reactions.
Reduced Molecular Weight Determination Methods and Procedures
Creative Proteomics uses enzymatic removal of glycans from antibody molecules, followed by accurate determination of the molecular weight of the antibody drug after deglycosylation by our advanced platform technology. The specific steps include the following:
We require a small quantity of the antibody drug sample for the assay. Our team of experts will guide you through the collection and preparation process to ensure accurate and reliable results.
- Enzymatic deglycosylation
For the removal of glycans, we employ specific enzymes that cleave the glycan chains from the antibody molecule while leaving the protein backbone intact. This enzyme treatment is carefully optimized to obtain complete glycan removal without affecting the structural integrity of the antibody.
- Molecular weight analysis
After deglycosylation, the molecular weight of the antibody drug is determined using advanced techniques such as size exclusion chromatography (SEC), mass spectrometry (MS), or high-performance liquid chromatography (HPLC). These methods provide precise measurements and allow for the identification of any changes in the molecular weight profile due to glycan removal.
Benefits and Applications of Accurate Deglycosylated Molecular Weight Determination
By choosing our deglycosylated molecular weight determination services for antibody drugs, you can benefit from the following advantages:
- Accurate determination of molecular weight
Deglycosylated molecular weight determination is an important component of the complete molecular weight determination of antibody drugs. The removal of sugars provides a more accurate measurement of the molecular weight of a pure protein or peptide, contributing to a better understanding of the structure and function of the antibody drug and assessing the purity of the protein.
Deglycosylated molecular weight testing can be utilized as a quality control measure in the production of pharmaceuticals and biologics. By ensuring the accurate determination of molecular weight, it helps in verifying the consistency and quality of the products.
- Assessment of protein-protein interactions
Understanding the interactions between proteins is fundamental in deciphering various biological processes. By accurately defining the molecular weight of individual proteins, deglycosylation can aid in studying protein-protein interactions, providing insights into complex cellular mechanisms.
Through this service, Creative Proteomics aims to assist customers in ensuring the quality and efficacy of their antibody drugs. Contact us to learn more about deglycosylated molecular weight assays for antibody drugs. We will be happy to assist you.
