CDC Activity Testing for Antibody Drugs is vital for evaluating the efficacy and safety of therapeutic antibody drugs. Complement-dependent cytotoxicity (CDC) is a mechanism by which antibodies can directly kill targeted cells. At Creative Proteomics, we offer comprehensive CDC testing services to assist pharmaceutical companies and researchers in assessing the effectiveness of antibody drugs in combating diseases. With our cutting-edge technology and vast experience in cell-based assays, we provide valuable insights that help accelerate the development of antibody drugs and improve patient outcomes.
Why is CDC Activity Testing Necessary?
Antibodies can mediate killing of target cells by binding to antigens on the cell surface and activating complement dependent cytotoxicity (CDC). Detecting whether an antibody can mediate CDC and how strongly it mediates CDC activity is a critical step in the development of antibody drugs and their quality control. Testing the CDC activity of antibody drugs provides critical data on efficacy, mechanism of action, safety and quality, ultimately contributing to the development and approval of safe and effective therapeutics.
Fig. 1 Antibody-mediated complement activation. (Van, Erp. E. A. et al., 2019)
Our CDC Testing Services
- Complement-mediated lysis assay
Our highly proficient team employs state-of-the-art techniques to perform complement-mediated lysis assays, assessing the ability of antibodies to induce CDC. This assay entails incubating labeled target cells with tested samples, complement, and test antibodies, followed by quantification of cell lysis via flow cytometry. By measuring the level of cell death, we determine the cytotoxic effects of the tested antibodies.
- ADCC and CDC combination assay
In certain instances, it is beneficial to assess both antibody-dependent cellular cytotoxicity (ADCC) and CDC simultaneously. Our experts conduct assays to evaluate the combined effects of these two mechanisms, providing a comprehensive understanding of antibody functionality. This approach ensures a more comprehensive evaluation and enables the selection of antibodies with optimal therapeutic potential.
CDC Testing Service Features
We recognize that each project is unique, and our team is committed to tailoring our services to meet your specific requirements.
We utilize the latest advancements in cell-based assays and flow cytometry to provide accurate and reliable results. Our advanced instrumentation ensures the highest level of sensitivity and specificity, allowing us to precisely measure CDC activity for antibody drugs.
We understand the importance of timely results. Our efficient processes allow for timely delivery of results, helping you make informed decisions in a timely manner.
Creative Proteomics' CDC activity assay service provides a comprehensive, customizable solution for assessing the potency of antibody drugs. With our expertise and state-of-the-art equipment, we aim to provide reliable and efficient antibody detection services. Contact us to learn more about how our antibody drug CDC activity testing service can contribute to the success of your drug development efforts.
Reference
- Van, Erp. E. A.; et al. Fc-mediated antibody effector functions during respiratory syncytial virus infection and disease. Frontiers in immunology. 2019, 10: 548.
