ADCs Coupled Site Analysis
Online Inquiry
For antibody drug conjugates (ADCs), characterization of the coupled site of the drug on the antibody and its coupled ratio is a critical step for quality control. During the production of antibody drug conjugates, the process of coupling the payload to the antibody is prone to nonspecific coupling, resulting in heterogeneous mixtures of ADCs with varying amounts and coupled sites. Since drug coupled sites and levels can have a significant impact on both physical and drug properties of ADCs. Therefore, reliable and straightforward methods for analyzing ADC coupled sites are needed. Creative Proteomics employs advanced mass spectrometry techniques to identify coupled sites and measure coupled levels to help customers gain a deeper understanding of payload binding efficiencies, optimize ADC development, and improve drug loading efficiency and efficacy.
Significance of ADCs Coupled Site Analysis
The coupling site analysis involves identifying and characterizing the specific sites on the antibody where the drug payload can be attached. Accurate and effective coupling site analysis is critical to the development of antibody drug conjugates for several key reasons including:
- Evaluates the precision and controllability of drug attachment to ensure efficacy and minimize off-target effects of ADCs.
- Determine the optimal drug-antibody ratio, which will affect the pharmacokinetics, stability, and therapeutic window of the ADCs.
- The coupling step is a critical step in the development of antibody drug conjugates to ensure safety, efficacy and clinical success.
Our ADCs Coupled Site Analysis Services
Our highly skilled team specializes in the analysis of antibody drug conjugates (ADCs) and offers a range of services to thoroughly characterize and identify the coupling sites of ADCs.
We use advanced analytical techniques to perform detailed analysis of the coupling sites between the antibody and the drug payload, accurately identifying and characterizing the specific sites where the drug and antibody are coupled.
- Characterization of linker-payload stability
We assess the stability of the linker-payload bond at the coupling site, providing critical insights into the stability and release kinetics of the payload.
- Analysis of conjugation efficiency
Our skilled scientists carefully evaluate the coupling efficiency of each coupling site to determine the level at which the payload attaches to the antibody, helping our customers analyze the consistency and effectiveness of their products.
Service Process
We are committed to providing customers with one-stop ADCs coupling site analysis services to help customers save valuable time. All you need to do is to submit your samples to us and our experts will work closely with you to customize a test plan that matches the characteristics of your samples and deliver a comprehensive report summarizing the results of your coupled-site analysis. Below is an overview of our service process:
The target antibody drug conjugate is extracted from the sample to be tested to ensure its isolation and purity.
Enzymatic digestion of prepared samples breaks down the conjugate into smaller peptides to facilitate further analysis and identification of coupling sites.
- LC-MS/MS mass spectrometry analysis
Digested peptides are analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Our highly sensitive and accurate analytical techniques allow accurate analysis and identification of coupling sites.
Our expert team uses advanced data analysis methods to interpret LC-MS/MS results. This includes peptide mapping, identification of coupling sites, determination of drug-antibody ratios (DAR) and characterization of any modification or degradation products.
We ensure that a comprehensive analysis report, including detailed information on the identified coupling sites, is delivered to our clients in the shortest possible time.
Advantages of Our ADCs Coupled Site Analysis
- Extensive analytical capabilities allow comprehensive analysis of ADCs coupling sites, including chemical and enzymatic coupling methods.
- Fully consider factors such as fragmentation of the payload-linker in mass spectrometry.
- Optimize the data analysis process to derive the exact payload binding site.
Creative Proteomics helps clients optimize the ADCs development process through detailed analysis of coupling sites, reducing the time required for product development, stability studies and regulatory filings. Contact us to learn more about our service and we will be happy to serve you.
