Glycosylation Site Analysis

Glycosylation modifications play a crucial role in protein folding, stability and functionality. Glycosylation site analysis is a powerful tool that allows researchers to identify and analyze glycosylation sites on proteins of interest. At Creative Proteomics, we offer comprehensive glycosylation site analysis services using state-of-the-art technology and analysis systems. Our team of experienced scientists is committed to delivering accurate and reliable results to meet the needs of your specific stage of development.

Our Glycosylation Site Analysis Services

Creative Proteomics utilizes cutting-edge technologies such as mass spectrometry, liquid chromatography, and advanced bioinformatics tools to perform in-depth glycosylation site analysis. Our team of scientists has extensive experience in glycoproteomics, enabling us to deliver accurate and reliable results.

• N-glycosylation site analysis services

N-glycosylation involves the attachment of complex sugar chains (glycans) to the nitrogen atom of the asparagine (N) residue within the protein sequence. By analyzing N-glycosylation sites, we can determine the type and diversity of glycans attached, as well as their distribution across the antibody molecule. This analysis helps in understanding the antibody's function, half-life, immunogenicity, and other critical properties.

• O-glycosylation site analysis services

We focuse on the analysis of glycans attached to the hydroxyl group of the serine (S) or threonine (T) residues. O-glycosylation plays a significant role in antibody stability, cellular localization, and intermolecular interactions. By examining O-glycosylation sites, we can provide valuable insights into the specific glycan structures involved and their impact on antibody functionality.

Processes of Our Glycosylation Site Analysis Services

• Enzymatic digestion

Select the appropriate protease to enzymatically digest the sample, covering the target protein sequence as completely as possible.

• Chromatographic separation

Separation of N-glycopeptides and O-glycopeptides by chromatographic columns.

• Deglycosylation
• Peptide analysis

Peptide analysis by MALDI-TOF MS or ESI-MS/MS.

• Analysis of N/O-glycosylation sites based on mass spectrometry results.

Application Fields

• Identification of glycoforms corresponding to N-glycosylation sites in glycosylated proteins.
• Identification of glycoforms corresponding to O-glycosylation sites in glycosylated proteins.

Advantages of Our Service

When you choose our glycosylation site analysis services, you can expect:

• Comprehensive analysis of N-glycosylation and O-glycosylation sites in your antibody drugs.
• Detailed reports outlining the specific glycan structures identified and their distribution.
• Insights into the functional implications of identified glycosylation sites.
• Recommendations for optimizing antibody glycosylation profiles to enhance therapeutic efficacy and safety.
• Fast turnaround times and results delivery.

What We Deliver?

Creative Proteomics has extensive experience in glycosylation analysis and can provide our clients with customized solutions and timely project delivery. Contact us to learn more about our glycosylation site analysis services of antibody drugs. We will be happy to assist you.