Sedimentation Equilibrium Analytical Ultra Centrifugation (SE-AUC)
Online Inquiry
Analytical ultracentrifugation (AUC) is a critical analytical tool supporting the development and manufacture of antibody therapeutics. Sedimentation equilibrium analytical ultra centrifugation (SE-AUC) is an alternative mode of operation for analytical ultracentrifugation and has a powerful analytical capability that provides the most accurate information on the (concentration-dependent) apparent molecular weight of the species in solution. Creative Proteomics offers the latest SE-AUC technology equipped with best-in-class assays. We have used the technology to focus on issues surrounding its application in the characterization of antibody drug aggregates and performed methodological validation. The results show that our SE-AUC technology has good precision and reproducibility, and can accurately provide key parameters related to antibody-drug aggregates, protein-protein interactions, and so on.
Introduction of Sedimentation Equilibrium Analytical Ultra Centrifugation (SE-AUC)
Sedimentation equilibrium analysis ultracentrifugation (SE-AUC) is a powerful analytical technique used in the characterization of antibody drugs. It allows for the determination of size, oligomeric state, and association constants of protein samples, including antibody drugs, in solution. SE-AUC works by subjecting the protein sample to high gravitational forces in an ultracentrifuge. As the sample undergoes sedimentation, its distribution across the radial axis of the centrifuge cell is measured and analyzed. By analyzing the sedimentation behavior of the sample, valuable information about its size and composition can be obtained.
Application of SE-AUC in Antibody Drug Characterization
SE-AUC is a versatile assay that is often used in our lab to detect multiple aspects of antibody drugs, including:
- SE-AUC can accurately determine the size and aggregation state of an antibody drug.
- SE-AUC provides insight into the behavior of proteins in solution, which can assist in formulation development and stability assessment of drugs.
- SE-AUC monitors changes in pH, temperature, or formulation conditions and can evaluate the effect of various factors on the stability of antibody drugs.
- SE-AUC can provide valuable insights into the binding affinities and stoichiometry of antibody-drug interactions.
Advantages of SE-AUC
- Excellent resolution and sensitivity.
- A label-free technique.
- Fast and accurate testing.
- Multifaceted functional analysis of different types of antibody drugs.
Our Capabilities
- With our efficient SE-AUC analysis tools, we can perform detailed characterization of different aspects of different types of antibody drugs.
- Customized services to meet the specific needs of all clients.
- Comprehensive analytical reports providing in-depth information on molecular weight, aggregate content, impurities, and stability of antibody drugs.
Creative Proteomics offers state-of-the-art SE-AUC instrumentation and expertise to support antibody drug characterization. With our SE-AUC services, you can gain comprehensive insights into the physicochemical properties of your antibody drug, aiding in its development and ensuring its quality. Contact us to learn more about our service and we will be happy to serve you.
