Peptide Mapping

Peptide map analysis is a technique widely used to examine the primary structure of antibody drugs. Creative Proteomics will develop a protocol to enzymatically digest the sample according to the customer's needs, and the peptides will enter mass spectrometry for detection, and the detailed information of the corresponding peptides in the peptide map will be analyzed and characterized using advanced software.

Introduction of Peptide Mapping Service

Antibody drugs have complex structures that often include the presence of various disulfide bonds and post-translational modifications. Peptide map analysis is a key workflow in antibody drug characterization and has the advantage of providing specific information on post-translational and chemical modifications, which is essential for resolving the primary amino acid structure of proteins. In addition, peptide maps comparing different batches of products can verify the stability of the process and ensure the quality and purity of the product. Our professional peptide map analysis services are a key quality control tool in antibody drug development, manufacturing and regulatory evaluation, effectively helping our clients ensure the safety and efficacy of these important therapeutic agents.

Peptide Mapping Technical Process

Creative Proteomics experienced scientists sequence peptides by using a variety of proteolytic reactions and advanced mass spectrometers. Using our proprietary analysis software, mass spectrometry data can be analyzed for any non-specific protease digestion product. We guarantee 100% sequence coverage as well as high precision sequence confirmation. Specific processes include the following:

• Protein digestion

To first stage in peptide mapping is to break the protein down into peptide fragments by proteolytic enzymes. We perform enzymatic cleavage and identification of target proteins using enzymatic methods according to our customers' samples and needs to ensure maximum sequence coverage and sensitivity. Our commonly used enzymes and cleavage sites include the following:

• Sample analysis

The samples were then analyzed using LC-MS/MS or UV LC-MS/MS. MS has become an important method of detection for characterizing peptides and peptide maps, making it possible to quickly determine the identity of peptide fragments.

• Comparison analysis

Peptides can be analysed through direct infusion of the isolated peptides, or by the use of online LC/MS. The results can be correlated to the protein, to confirm the specific amino acid sequences covered by the peptide map and the protein identity. By comparing the measured masses from the MS information to the predicted values from the intact protein or protein database, the mass and sequence coverage information can be used to identify the protein.

Peptide Mapping Advantages of Creative Proteomics

• Depending on your antibody drug type, custom methods optimized to fit your samples are provided and ensure maximum information is obtained for you from the technique.
• The multi-protease combination for sample pre-treatment ensures up to 100% coverage of the peptide map.
• Advanced analytical techniques provide detailed information on the corresponding peptides, including sequence, post-translational modifications, mutations, etc.

At Creative Proteomics, we can provide professional and efficient peptide map analysis services for primary structural characterization of antibody drugs, as well as advice and support for method development and process optimization through our in-house technical team. Contact us to learn more about peptide mapping for antibody drugs. We will be happy to assist you.