Flavonol Analysis Service
Service Details Case Study

Why Need Flavonols Analysis?

Flavonols are a class of flavonoids, a group of polyphenolic secondary metabolites naturally produced in many fruits, vegetables and medicinal herbs. Flavonols, encompassing compounds such as Quercetin, Kaempferol and Myricetin, are known for their significant antioxidant, anti-inflammatory and anti-cancer properties.

The antioxidant capacity of flavonols comes from their ion chelating ability and radical scavenging activity, which facilitates the disruption of free radical chains. This antioxidant activity is a cornerstone of the health-promoting benefits of flavonols, including their potential role in prevention and treatment of various chronic diseases such as cardiovascular and neurodegenerative diseases, and cancers.

The significance of flavonols analysis lies in their extensive health-promoting properties. By analyzing the flavonol profile of specific foods or supplements, we can unravel their potential health benefits and use this knowledge in the nutraceutical industry to develop products with health-promoting properties. Furthermore, flavonols analysis in medicinal herbs and plants assists in understanding the therapeutic potential of these resources.

Additionally, the analysis of flavonols in plants provides essential insights into plant physiology. Flavonols play vital roles in plant growth and development, such as UV-B protection, modulation of auxin transport, and response to nutrient deficiency. Therefore, flavonols profiling is equally important in agricultural practices, where it can provide information related to the adaptation, growth, and productivity of crops.

Flavonols Analysis Services by Creative Proteomics

At Creative Proteomics, our dedicated team of experienced scientists provides a comprehensive suite of flavonols analysis solutions. Leveraging state-of-the-art techniques and advanced technologies, such as liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) spectroscopy, we cater to the diverse research needs and deliver reliable results with high sensitivity and accuracy.

Our analysis services include but are not limited to the following:

  • Flavonols Profiling: This service provides a detailed overview of the flavonol compositions in different samples. By employing advanced analytical techniques like UHPLC and Q-TOF MS/MS, we ensure accurate identification and quantitation of various flavonols.
  • Targeted Flavonols Analysis: If you are mainly interested in specific flavonols, we customize our analysis based on your specific objectives, providing precise and quantitative results on your targeted flavonols.
  • Flavonols Metabolomics Study: With the vast array of technologies and methodologies at our disposal, we can carry out a comprehensive metabolomics study of flavonols in various biological samples. Our metabolomics study assists you in understanding the metabolic pathways and the physiological roles of flavonols.
  • Structure Identification Service: Through mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy, we provide service for the structure identification of flavonols.

With a customer-centric approach, we strive to accelerate your research and provide substantial information that contributes to the advancement of knowledge in flavonols and their significant roles in botany and human health.

Workflow for Plant Metabolomics ServiceWorkflow for Plant Metabolomics Service

List of Flavonols Analyzed (including but not limited to)

Kaempferol Quercetin Myricetin Fisetin Rutin
Morin Isorhamnetin Hesperetin Naringenin Apigenin
Luteolin Genistein Daidzein Gallocatechin Epigallocatechin
Catechin Epicatechin Proanthocyanidin Anthocyanidin Cyanidin
Delphinidin Pelargonidin Petunidin Peonidin Malvidin
Astragalin Pachypodol Rhamnazin

Sample Requirements for Flavonols Assay

Sample Type Recommended Volume
Fresh Plant Tissues 1-5 grams or 5-10 mL
Dried Plant Material 1-5 grams
Fruit Juices 10-20 mL
Wine 10-20 mL
Tea Leaves 1-5 grams or 5-10 mL
Honey 10-20 grams
Blood Serum 0.5-1 mL
Urine 0.5-1 mL
Tissues (Human or Animal) 20-50 mg
Dietary Supplements 5-10 grams or 10-20 mL
Pharmaceuticals As per product specifications (typically 5-20 grams)

Deliverables of Flavonols Analysis

  • Analysis Report: A detailed report outlining the methodology, instrumentation used, and results obtained from the flavonols analysis.
  • Quantification Results: Accurate quantification of individual flavonols present in the samples, expressed in appropriate units.
  • Chromatograms: Chromatographic profiles showcasing the separation of flavonols and other relevant compounds.
  • Structural Elucidation (if applicable): Detailed information on the structure of flavonols identified in the samples.
  • Customized Data Analysis (if requested): Tailored data analysis based on specific research objectives or parameters.

Case. Polyphenolic Composition and Maturation-Dependent Changes in Pistacia lentiscus Fruits


Pistacia lentiscus fruits exhibit dynamic changes in polyphenolic composition during maturation. Understanding these variations is crucial for potential applications in the food industry and nutraceutical development.


The study focused on P. lentiscus fruits collected at five different maturation stages: September 2017 (less red unripe fruits), October 2017 (red unripe fruits), November 2017 (red unripe fruits), December 2017 (majority black ripe fruits), and January 2018 (black fully ripe fruits).

Technical Platform and Procedure:

Polyphenolic compounds were analyzed using a targeted UHPLC-QqQ-MS/MS approach. Anthocyanins were identified using UHPLC-UV/vis-MS/MS with a method optimized for separation. Various scan modes, including full MS, MS/MS fragmentation analysis, precursor ion scan, and neutral loss scan, were employed for identification. Additional analysis involved UHPLC-QqQ mass spectrometry for other phenolic compounds. Statistical analysis was performed using one-way ANOVA tests.


The study identified 30 compounds, including 9 anthocyanins, in P. lentiscus fruits. Anthocyanin composition comprised 3 cyanidin derivatives and 6 delphinidin derivatives with various glycoside moieties. Delphinidin galactoside, quercetin galactoside, and quercetin glucoside were major compounds among anthocyanins and flavonols. Gallocatechin dominated the flavanol group. Protocatechuic acid and trans-piceid were quantified for the first time.

Polyphenol content varied significantly during maturation. Flavonols and flavanols were predominant in early stages, decreasing as fruits ripened. Anthocyanins sharply increased in mature fruits, making them the main polyphenols, offering potential dietary and nutraceutical applications.

UHPLC-DAD chromatogram of Pistacia lentiscus anthocyanins.UHPLC-DAD chromatogram of Pistacia lentiscus anthocyanins.


  1. Aissat, Aghiles Karim, et al. "Analysis of individual anthocyanins, flavanols, flavonols and other polyphenols in Pistacia lentiscus L. fruits during ripening." Journal of Food Composition and Analysis 106 (2022): 104286.
Plant Metabolomics Analysis