Ultra-deep and Quantitative Saliva Proteomics Solutions

Body fluids like blood, saliva, tears, sweat, urine, and cerebrospinal fluid are a source of putative biochemical markers that reflect various pathophysiological disorders. The saliva secreted from salivary glands and gingival crevice has some components that originate from the plasma with an overlap of almost 20–30% with plasma proteome, and the majority of these exhibit antimicrobial activity, transport, and enzymatic functions. Human saliva, therefore, is a potential diagnostic fluid that can reflect many pathophysiological states of the body.

In addition, saliva testing is a non-invasive and inexpensive assay. For the reasons above, mass spectrometry-based salivary proteomics can be a powerful tool for screening biomarkers of oral diseases. The salivary proteome has been characterized in several diseases: oral squamous cell carcinoma and oral leukoplakia, chronic graft-versus-host disease Sjögren's syndrome and other autoimmune disorders such as SAPHO, schizophrenia and bipolar disorder, and genetic diseases like Down's Syndrome and Wilson disease.

Our Salivary Proteins Quantitative Service

We analyze saliva proteins using advanced DIA-based discovery proteomics technology to reveal a large amount of disease-related functional information in high-throughput mode for a large number of saliva samples, helping you identify novel biomarkers associated with related diseases. We also offer highly multiplexed targeted proteomics with absolute quantification for customized panels of proteins.

Workflow

The depth and relatively straightforward nature of our workflow should make it a powerful new tool in the detection of biomarkers of oral diseases.

Label-free quantitative discovery and MRM based targeted validation workflow.

Data Analysis

Advantages

• High accuracy: QC CV as low as 25% for large projects
• High throughput, thousands of proteins can be quantified at once
• Quantitatively identify nearly all detectable molecules, covering low-abundance proteins/peptides
• High repeatability, increased by 30% and up to 90%
• Complete and comprehensive sample information storage in the first analysis
• Standard data and advanced data analysis
• Cost-effective

Sample Requirements

Report

• Experimental steps
• Relevant experiment parameters
• Mass spectrometry spectra
• Raw data
• Proteomics analysis results

Since we provide DIA technology service, we have accumulated a lot of experience not only detecting a large cohort of conventional samples, but also providing one-stop service of DIA+PRM+machine learning for biomarker studies, tumor-based proteomic typing and large clinical sample proteomics analysis based on next-generation chromatography (Evosep DIA). Please contact us for a free consultation.

References:

1. Niklas Grassl, et al. Ultra-deep and quantitative saliva proteome reveals dynamics of the oral microbiome. Genome Medicine 8 44 (2016)
2. M.Castagnola, et al. Salivary biomarkers and proteomics: future diagnostic and clinical utilities.
3. Acta Otorhinolaryngol Ital37 2: 94–101 (2017)
4. Amit Kumar Dey, et al. Salivary proteome signatures in the early and middle stages of human pregnancy with term birth outcome. Scientific Reports 10,8022 (2020)