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4D-Glycosylation Proteomics Services

Glycosylation is the process of transferring glycan to proteins under the action of glycosyltransferases to form glycosidic bonds with amino acid residues. Glycosylation is an important protein modification and plays a crucial role in the proper folding, functional localization, and intracellular transport of proteins.

Among all glycosylation modifications, N-glycosylation is one of the most widely studied cases. N-glycosylation involves in the essential role of sugar chains in various life activities, including cell recognition, cell differentiation, signal transduction, and immune response by linking with the free asparagine's NH2 group of proteins. Moreover, N-glycosylation is significant in the research of embryonic development, cancer development, and immune defense and defense against pathogenic bacteria invasion.

types of n-glycans

Our 4D-Glycosylation Proteomics Services

Creative Proteomics uses the novel generation of 4D platform to provide qualitative and quantitative analysis for protein glycosylation and modification sites, which significantly improves sensitivity and depth of coverage. The mass spectrometry technique and enrichment processing allow for large throughput detection of glycosylation-modified proteins with low levels and a wide dynamic range. We provide "one-stop" proteomics services, from large-scale PTMs proteomics screening, PRM validation of targeted proteomics/PTMs omics, to high-quality antibody-assisted validation and subsequent molecular mechanism studies.





Data Analysis

Standard Data Analysis
Analysis of total protein identification quantityProtein identification and quantitative result statistics, Venn diagram analysis, number of different results statistics, volcano map, cluster heat map
Protein function analysisSubcellular localization, domain analysis, motif analysis, GO function analysis, KEGG pathway analysis, interaction network analysis
Quality control analysisPeptide relative molecular mass distribution, peptide sequence length distribution

Sample Requirements

Cell: 5×107-108
Protein: >800 µg
Cell collection (try to avoid trypsin); phosphatase inhibitors are required throughout sample processing; protein samples are transported on dry ice.





  1. Quantitative chemoproteomics reveals OGlcNAcylation of cystathionine g-lyase (CSE) represses trophoblast syncytialization. 2021.Cell chemical biology.
  2. A mutant O-GlcNAcase enriches Drosophila developmental regulators. 2017.Nature chemical biology.
  3. The Protein Modifications of O-GlcNAcylation and Phosphorylation Mediate Vernalization Response for- Flowering in Winter Wheat. 2019. Plant physiology.
* For Research Use Only. Not for use in the treatment or diagnosis of disease.

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Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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