Immunopeptidome Profiling Service

The immunopeptidome refers to the study of all peptides presented by nucleated cells on MHC-Class I or II proteins (Major Histocompatibility Complex, also known as the major histocompatibility complex, such as the expression product of human MHC, HLA, Human Leukocyte Antigen) involved in the antigen presentation pathway, and is essential for the investigation of immunogenic epitopes in cell-mediated and humoral responses.

Immunopeptidome profiling is the study of the dynamics and composition of class I and II immune peptides, enabling not only the discovery of targets for personalized cancer immunotherapy (such as neoantigens from tumor-specific mutations or tumor-associated antigens), but also contributes to new mRNA/polypeptide vaccines and immune cell therapy.

Our Immunopeptidome Profiling Service

Creative Proteomics offers a one-stop solution for immunopeptidomic analysis and neoantigen discovery based on high-precision mass spectrometry, including our proprietary, highly sensitive immunopeptide enrichment and identification solutions. We are capable of identifying and characterizing more than 10,000 type I peptides and more than 10,000 type II peptides. Reproducible identification and quantification from minimal sample material is possible with our optimized high-throughput immunopeptidomics analysis platform. The service can be applied to large-scale studies aimed at finding solutions for cancer, immune diseases and infectious diseases, digging deeper into unknown targets.

Advantages of Our Immunopeptidome Profiling Service

1, mass spectrometry-based peptide identification method can identify to immune peptides more directly and accurately.

2, Mass spectrometry de novo sequencing technology, which does not depend on existing databases, can identify more atypical immune peptides.

3, The potential post-translational modifications of immune peptides can be analyzed.

Immunopeptidmics analysis and neoantigen discovery analysis strategy based on high precision mass spectrometry (Nature Biotechnology 40, 175–188 (2022))

The Role of Neoantigens in Immunotherapy

Catherine J Wu, et al. Nature.2017   Baorui Liu, et al. J Clin Invest.2019

Neoantigens Prediction

Schumacher TN, Schreiber RD. Science.2015

Advantages and Disadvantages of Neoantigen Prediction

Advantages:

Neoantigen prediction requires only sequencing results

Prediction software is easy to use and a large number of online tools are available

Disadvantages:

List of hundreds to thousands of candidate neoantigenic peptides, only a few of which are immunogenic

Validation and screening are time-consuming and labor-intensive

Whether the identified neoantigens are actually presented on the tumor surface is unknown

One-stop Solution for Neoantigen Discovery

Our one-stop solution for neoantigen discovery, including peptide synthesis, functional screening of neoantigen candidates.

Delivery

1. List of all identified immune peptides, neoantigenic peptides and tumor-associated antigenic peptides

2. data analysis report

Service Process

1. Case analysis

Ensure fully understand your needs

Professional technology platform to provide you with quality analysis strategies and perfect solutions

Our analysis strategy, operation process, and experienced team can provide you with a comprehensive and customizable solution according to your project needs

2. Project

The project manager will follow up your project throughout the process and provide one-stop analysis solutions

Strict Quality Control

3. Results Delivery

Standard project reports

Detailed study reports and test procedures can be provided according to project requirements

Sample Requirement