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Data-Independent Acquisition (DIA) Proteomics

Accuracy: High-resolution mass spectrometry for precise results.
Stability: 1 ppm accuracy over time, minimal corrections.
Sensitivity: Detects low-abundance proteins (amol levels).
Efficiency: Monitors all proteins at once, faster analysis.
Speed: Rapid 12-20 Hz scanning.
Range: Wide dynamic range for comprehensive quantification.
Selectivity: Enhanced with msxDIA technology.

Reliable Identification: dia-PASEF enhances sensitivity and selectivity.
Speedier Analysis: TIMS separation accelerates while maintaining accuracy.
Comprehensive Coverage: Deeper insights with extensive proteome coverage.
High-Sensitivity Targeting: prm-PASEF boosts target monitoring without compromise.

FFPE Quantitative Proteomics Solutions

At present, the majority of current clinical proteomics studies are still carried out on fresh or frozen tissue samples, which often lack sufficiently long follow-up information, limiting their practical applications. On the other hand, Formalin Fixed Paraffin-Embedded (FFPE) has been the most commonly used method of sample preservation for the clinical histopathological diagnosis of diseases. FFPE also is an economical archival choice, as tissues can be stored at a great density at room temperature (RT) for years or decades while maintaining integrity for pathology analyses. An estimated 500 million FFPE cancer tissues are stored to date, and this number is rapidly rising. These present an invaluable resource for studying molecular mechanisms underlying diseases and testing potential biomarkers and discovering new ones. If these samples can be applied to proteomic analysis, they will undoubtedly be of better clinical value. The recent technological progress of MS-based proteomic methods now enables the unbiased and large-scale investigation of proteomes at ever greater depth.

Our FFPE Quantitative Proteomics Service

We apply advanced DIA proteomics analysis technology to FFPE tissue protein extracts, which can reveal a large amount of disease-related functional information, so as to help you identify meaningful biomarkers related to the diagnosis, prognosis, monitoring and treatment of specific diseases. We also offer highly multiplexed targeted proteomics with absolute quantification for customized panels of proteins.

Mass spectrometry-based workflow for FFPE tissue analysis (Fabian Coscia, et al, bioRxio. 2020)

Help You Overcome Technical Difficulties

Tissue Treatments such as formaldehyde fixation and paraffin embedding lead to extensive molecular cross-linking of proteins, nucleic acids and other molecules in tissue cells, making it quite difficult to obtain proteome within FFPE tissues. Formaldehyde fixation limits the extraction efficiency of proteins, reduces the immune response activity, and may lead to unclear identification of proteins by mass spectrometry. Therefore, an efficient method for protein extraction from FFPE tissues is needed. Based on this, Creative Proteomics has developed an extraction method that can obtain high-quality proteins from FFPE samples after extensive literature research and R&D testing.

Data Analysis

Standard data analysis content
Statistical analysis of identification results	Sample protein and peptide identification histogram, PCA distribution map, quantitative variance statistical analysis
Bioinformatics analysis	Differential up- and down-regulated protein KEGG map, KEGG pathway function attribution, etc.
Bioinformatics analysis	GO and G0 enrichment, KEGG and KEGG enrichment analysis, PPI interaction network and module analysis
Advanced data analysis content
Biomarker screening	Integrated machine learning, LASSO regression analysis, marker panels, ROC analysis
Molecular typing analysis	Unsupervised cluster typing analysis, proteome + transcriptome analysis, kinase analysis
Joint analysis of clinical characterization and omics results
Disease-related functional module analysis	WGCNA co-expression analysis
Survival curve analysis	Clinical typing and survival curve analysis

Advantages

High accuracy: QC CV as low as 25% for large projects
High throughput, more than 9000 proteins can be identified at once
Quantitatively identify nearly all detectable molecules, covering low-abundance proteins/peptides
High repeatability increased by 30% and up to 90%
Complete and comprehensive sample information storage in the first analysis
Standard data and advanced data analysis
Low cost: reduce the cost by more than 3 times

Sample Requirements

Sample Type	Protein	# of Cell	FFPE slices
Quantify	100 μg	1×10⁷ cells	2×5 μm FFPE slices (each 50-100mm²)

Report

Experimental steps
Relevant experiment parameters
Mass spectrometry spectra
Raw data
Proteomics analysis results

Our FFPE Quantitative Proteomics Service is ideal for long-term projects or projects with large sample sets that require accurate and reproducible quantitation. Please contact us for more details.

Reference:

Fabian Coscia, et al., 2019, A streamlined mass spectrometry-based proteomics workflow for large scale FFPE tissue analysis. bioRxio. 251(1):100-112. 2020

* For Research Use Only. Not for use in the treatment or diagnosis of disease.

ICON 4D Proteomics with Data-Independent Acquisition (DIA)

50% detection rate of low-peak intensity proteins
Higher integrity and reproducibility of protein data
Nearly 100% ion utilization, greatly improving detection sensitivity
Fewer injections, shorter gradients, and deeper coverage
Stronger support for PTM identification

Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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