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Data-Independent Acquisition (DIA) Proteomics

Accuracy: High-resolution mass spectrometry for precise results.
Stability: 1 ppm accuracy over time, minimal corrections.
Sensitivity: Detects low-abundance proteins (amol levels).
Efficiency: Monitors all proteins at once, faster analysis.
Speed: Rapid 12-20 Hz scanning.
Range: Wide dynamic range for comprehensive quantification.
Selectivity: Enhanced with msxDIA technology.

Reliable Identification: dia-PASEF enhances sensitivity and selectivity.
Speedier Analysis: TIMS separation accelerates while maintaining accuracy.
Comprehensive Coverage: Deeper insights with extensive proteome coverage.
High-Sensitivity Targeting: prm-PASEF boosts target monitoring without compromise.

4D-Phosphoproteomics Services

Protein phosphorylation modification is one of the most important covalent modifications in living organisms. It is the most distributed and studied modification which occurs by transferring the phosphate group of ATP to the amino acid (Ser, Tyr, Thr) side chain of proteins catalyzed by kinase. The reversible process of phosphorylation and dephosphorylation regulates all life activities, including cell proliferation, development, differentiation, signal transduction, apoptosis, neural activity, muscle contraction and tumorigenesis. Statistically, the molecular mass of phosphorylated amino acids covalently attached to the phosphate group increased by 79.97 Da. Thus, mass spectrometry can be used to accurately determine whether a mass shift of 79.97 Da has occurred in the molecular weight to detect phosphorylated peptides and modification sites. D-label-free phosphorylated proteomics is based on a new generation of timsTOF Pro mass spectrometer for differential phosphorylation proteomics analysis.

The catalytic cycle for protein phosphorylation by a protein kinase (Te Li 2019)

Our 4D-Phosphoproteomics Services

Creative Proteomics provides 4D-label free phosphoproteomics, using the ion flow separation to effectively distinguish modified tautomeric peptides according to the molecule's shape and cross-sectional area properties. This technology significantly improves the depth of modifications identification and achieve a comprehensive improvement of proteomics in terms of coverage depth, sensitivity and throughput with less loading volume and faster scanning speed.

Workflow

Applications

Plant development and resistance to stress.
Infection and immunity research.
Aging regeneration and degenerative diseases.

Data Analysis

Standard Data Analysis
Analysis of total protein identification quantity	Protein identification and quantitative result statistics, Venn diagram analysis, number of different results statistics, volcano map, cluster heat map
Protein function analysis	Subcellular localization, domain analysis, motif analysis, GO function analysis, KEGG pathway analysis, interaction network analysis
Quality control analysis	Peptide relative molecular mass distribution, peptide sequence length distribution
Advanced Data Analysis
Protein Gene Chromosome Localization	Obtain genes encoding proteins distribution on chromosomes
WGCNA Analysis	Predict functional clustering and network interactions by protein expression level; correlate with phenotype data to obtain key proteins or protein complexes that influence phenotype
Trend Cluster Analysis	Obtain protein expression trend patterns
Molecular Typing	Molecular typing for large cohort samples
Survival Curve Analysis	Study the relationship between influencing factors, survival time and outcome
ROC Curves	Evaluate predictive accuracy by combining specificity and sensitivity, such as biomarker impact assessment on tumor grade

Technical Advantages

The support of 4D technology improves quantification accuracy and identification depth, more suitable for trace samples;
Overcoming the limitation of labeled quantitative proteomics techniques in terms of sample size, which is flexible and convenient;
Cutting-edge technology-based on a new generation of mass spectrometry platform (Bruker timsTOF pro);
High speed - nearly doubled detection speed;
Extensive experience - over 10 years of protein platform service experience, with leading high-level project articles in quantity and quality;
Data assurance - stable and reliable data results;
"One-stop" service - PTMs analysis, verification, upstream and downstream joint analysis.

Sample Requirements

We can quickly extract proteins from various samples and design personalized experimental schemes according to different experimental purposes based on our unique protein extraction technology. Specific requirements are as follows:

Sample Type	Protein	# of Cells	Animal Tissue	Plant Tissue	Blood	Urine	Serum	Microbes
Quantify	100 ug	1×10⁷ cells	1 g	200 mg	1 mL	2 mL	0.2-0.5 mL	Dry weighed: 200 mg

Recommended Combination

(1) Proteome + Phosphorylation: comprehensive and precise dissection of regulatory networks.
(2) Phosphorylation + metabolomics: open up the phenotype-mechanism research pathway.

Reference:

Synergistically Bifunctional Paramagnetic Separation Enables Efficient Isolation of Urine Extracellular Vesicles and Downstream Phosphoproteomic Analysis. ACS Appl Mater Interfaces. 2021.

* For Research Use Only. Not for use in the treatment or diagnosis of disease.

ICON 4D Proteomics with Data-Independent Acquisition (DIA)

50% detection rate of low-peak intensity proteins
Higher integrity and reproducibility of protein data
Nearly 100% ion utilization, greatly improving detection sensitivity
Fewer injections, shorter gradients, and deeper coverage
Stronger support for PTM identification

Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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