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Data-Independent Acquisition (DIA) Proteomics

Accuracy: High-resolution mass spectrometry for precise results.
Stability: 1 ppm accuracy over time, minimal corrections.
Sensitivity: Detects low-abundance proteins (amol levels).
Efficiency: Monitors all proteins at once, faster analysis.
Speed: Rapid 12-20 Hz scanning.
Range: Wide dynamic range for comprehensive quantification.
Selectivity: Enhanced with msxDIA technology.

Reliable Identification: dia-PASEF enhances sensitivity and selectivity.
Speedier Analysis: TIMS separation accelerates while maintaining accuracy.
Comprehensive Coverage: Deeper insights with extensive proteome coverage.
High-Sensitivity Targeting: prm-PASEF boosts target monitoring without compromise.

Super Reaction Monitoring-SRM™ Quantitative Proteomics Services

Super Reaction Monitoring (SRM™) is a label-free next-generation proteomics technology platform established by Creative Proteomics. This platform combines Data-Dependent Analysis (DDA) and Data-Independent Acquisition (DIA) technologies to obtain data before each analysis. Shortcomings of DDA and selected reaction monitoring (SRM) in terms of depth and width that have limited researchers from examining complex biological systems are address by SRM™. The platform also allows an unmatched proteomics coverage with accurate and reproducible quantification of thousands of proteins per sample.

Construction of spectral database: Spectral database built from the DDA runs on the experimental samples or samples of interest. The spectral database is a non-redundant set of MS/MS spectra, serving as a template for peptide identification during data analysis.
Data collection in DIA mode: DIA experiments are conducted on a combined system with full scan data being recorded in a time-of-flight (ToF), Orbitrap or other high-resolution mass analyzers. Under the DIA model, nearly all detectable proteins (including low abundance proteins) can be quantitatively identified with high accuracy and repeatability.
Data analysis: The obtained data were matched and quantified by the accurate template peptide spectra from the spectral database. The most advanced algorithms are used in our OpenSWATH and Skyline software.

Tailored Solutions to Meet Your Needs

For large-scale proteomics projects: This is a standardized SRM™ platform with a special database built from the DDA and the fast turnaround time. Thousands of proteins can be duplicated and precisely quantified from one sample. This option is for customers looking for reliable and rapid solutions to quantify proteome changes in their samples.
For comprehensive proteomic analysis: Featuring an extended LC gradient for a deeper analysis and identification of more proteins, this option is for customers looking for an in-depth proteome quantification service.
For the most extensive proteome profiling research: Equipped with the most advanced high-resolution mass spectrometer instruments and an ultra-long LC gradient, this solution maximizes the depth of the analysis and identifies the highest numbers of proteins. This is for customers looking for projects with the highest proteome coverage on the market.
For rapid screening for large projects: We provide a unique solution for researchers in large studies with hundreds of samples who are looking for a reliable and fast service to quantify proteome changes on a large scale. This scheme features a shorter LC gradient which results in a faster turnaround time per sample.

Workflow

Super Reaction Monitoring Workflow

Analytical Platform

AB SCIEX Triple-TOF 5600-plus, Q-Exactive, Orbitrap Fusion

Bioinformatics Analysis

Problems to be Solved	Bioinformatics Analysis
Quality Assessment of Protein	DIA Data Analysis
Protein Comparison of Different Samples	Multivariate PCA Analysis
Protein Statistical Analysis	Venn Diagram
Protein Statistical Analysis	Volcano Plot
Functional Annotation	KEGG Annotation
	GO Annotation
	COG Annotation
Clustering Analysis	Hierarchical Clustering
Clustering Analysis	K-Means Clustering
Network Analysis	STRING Analysis

Key Features

Combine the advantages of DIA and DDA: based on DDA database and DIA analysis technology
High throughput: Identify and quantitate up to thousands of proteins at once
High accuracy and repeatability with second order mass spectrometry signal quantification and DIA based database
Quantitatively identify nearly all detectable proteins, including low abundance proteins

Sample Requirements

Based on our special protein extraction technology, we can quickly extract proteins from various samples and design personalized experimental schemes according to different experimental purposes. Specific requirements are as follows:

Sample Type	Protein	Cell	Animal Tissue	Plant Tissue	Blood	Urine	Serum	Microbes
Quantify	100 ug	1×10⁷ cells	1 g	200 mg	1 mL	2 mL	0.2-0.5 mL	Dry weighed: 200 mg

Note:

Please prepare enough dry ice or ice packs to ensure low temperature during sample delivery.
Our service is for research use only and is not intended for diagnosis.

Applications

Large-scale quantitative analysis of protein
Biomarker discovery and validation
Target discovery
Mechanism and regulation mechanism research
Pathological study of disease
Genetic association study
Microorganisms proteomic research
Crop proteomics research

Reports

Experiment report
Relative parameters of the mass spectrometer
MS raw data files
Bioinformatics analysis
Peptide/Protein identifications and intensities

Learn more about SWATH and MSX-DIA.

* For Research Use Only. Not for use in the treatment or diagnosis of disease.

ICON 4D Proteomics with Data-Independent Acquisition (DIA)

50% detection rate of low-peak intensity proteins
Higher integrity and reproducibility of protein data
Nearly 100% ion utilization, greatly improving detection sensitivity
Fewer injections, shorter gradients, and deeper coverage
Stronger support for PTM identification

Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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