SRM-MRM Quantitative Proteomics Services
Selected Reaction Monitoring (SRM), also known as Multiple Reaction Monitoring (MRM), is used to monitor several selected precursor-fragment transitions of the targeted peptides. The selection of the SRM/MRM transitions is calculated based on the acquired data. SRM/MRM, using a triple quadrupole MS, consists of three steps: selection of targeted precursor peptides based on MS spectra, fragmentation one (or more) of these precursor peptides, and acquisition fragment ions based on MS/MS spectra. Up to 6000 transitions can be monitored by SRM/MRM technology. The advantages of SRM/MRM include flexibility, sensitivity, specificity and reproducibility, making it ideal for quantitative analysis which enables the precise quantification of protein networks in biological systems. Furthermore, SRM/MRM based quantification can be combined with different strategies for relative or absolute protein quantification.
Workflow
Analytical Platform
AB SCIEX Triple TOF 5600，AB SCIEX Triple Quad™ 5500, Q-Exactive, Orbitrap Fusion
Bioinformatics Analysis
| Problems to be Solved | Bioinformatics Analysis |
| Quality Assessment of Protein | MRM Data Analysis |
| Protein Comparison of Different Samples | Multivariate PCA Analysis |
| Protein Statistical Analysis | Venn Diagram |
| Volcano Plot | |
| Functional Annotation | KEGG Annotation |
| GO Annotation | |
| COG Annotation | |
| Clustering Analysis | Hierarchical Clustering |
| K-Means Clustering | |
| Network Analysis | STRING Analysis |
Sample Requirement
Based on our special protein extraction technology, we can quickly extract proteins from various samples and formulate unique experimental schemes according to different research purposes. Specific requirements are as follows:
| Sample Type | Protein | Cell | Animal Tissue | Plant Tissue | Blood | Urine | Serum | Microbes |
| Quantify | 100 ug | 1×107 cells | 1 g | 200 mg | 1 mL | 2 mL | 0.2-0.5 mL | Dry weighed: 200 mg |
Note:
- Please prepare enough dry ice or ice packs to ensure low temperature during sample delivery.
- Our service is for research use only, and not for use in diagnostic procedures.
- Plasma sample measurement requires at least 10 µl of plasma.
Key Features
- High-throughput, and can identify up to 150 selected proteins at a time
- High accuracy, specificity and repeatability, and can identify low abundance proteins
- Broad dynamic range, covering 6 orders of magnitude of protein abundance at a time
- Absolute quantification of a variety of proteins without the need for antibodies
- No antibodies
Applications
- Biomarker discovery and validation
- Absolute quantitation: Absolute quantitation of proteins, peptides, metabolites and serum, lipids from plasma and other biological samples
- Protein post-translational modification analysis
- Verification of untargeted proteomics results
- Pathological study of diseases
Reports
- Experiment report
- Relative parameters of the mass spectrometer
- MS raw data files
- Bioinformatics analysis
- Peptide/Protein identification and intensity
4D Proteomics with Data-Independent Acquisition (DIA)