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Data-Independent Acquisition (DIA) Proteomics

Accuracy: High-resolution mass spectrometry for precise results.
Stability: 1 ppm accuracy over time, minimal corrections.
Sensitivity: Detects low-abundance proteins (amol levels).
Efficiency: Monitors all proteins at once, faster analysis.
Speed: Rapid 12-20 Hz scanning.
Range: Wide dynamic range for comprehensive quantification.
Selectivity: Enhanced with msxDIA technology.

Reliable Identification: dia-PASEF enhances sensitivity and selectivity.
Speedier Analysis: TIMS separation accelerates while maintaining accuracy.
Comprehensive Coverage: Deeper insights with extensive proteome coverage.
High-Sensitivity Targeting: prm-PASEF boosts target monitoring without compromise.

SRM-MRM Quantitative Proteomics Services

Selected Reaction Monitoring (SRM), also known as Multiple Reaction Monitoring (MRM), is used to monitor several selected precursor-fragment transitions of the targeted peptides. The selection of the SRM/MRM transitions is calculated based on the acquired data. SRM/MRM, using a triple quadrupole MS, consists of three steps: selection of targeted precursor peptides based on MS spectra, fragmentation one (or more) of these precursor peptides, and acquisition fragment ions based on MS/MS spectra. Up to 6000 transitions can be monitored by SRM/MRM technology. The advantages of SRM/MRM include flexibility, sensitivity, specificity and reproducibility, making it ideal for quantitative analysis which enables the precise quantification of protein networks in biological systems. Furthermore, SRM/MRM based quantification can be combined with different strategies for relative or absolute protein quantification.

Workflow

Selected/Multiple Reaction Monitoring Workflow

Analytical Platform

AB SCIEX Triple TOF 5600，AB SCIEX Triple Quad™ 5500, Q-Exactive, Orbitrap Fusion

Bioinformatics Analysis

Problems to be Solved	Bioinformatics Analysis
Quality Assessment of Protein	MRM Data Analysis
Protein Comparison of Different Samples	Multivariate PCA Analysis
Protein Statistical Analysis	Venn Diagram
Protein Statistical Analysis	Volcano Plot
Functional Annotation	KEGG Annotation
	GO Annotation
	COG Annotation
Clustering Analysis	Hierarchical Clustering
Clustering Analysis	K-Means Clustering
Network Analysis	STRING Analysis

Sample Requirement

Based on our special protein extraction technology, we can quickly extract proteins from various samples and formulate unique experimental schemes according to different research purposes. Specific requirements are as follows:

Sample Type	Protein	Cell	Animal Tissue	Plant Tissue	Blood	Urine	Serum	Microbes
Quantify	100 ug	1×10⁷ cells	1 g	200 mg	1 mL	2 mL	0.2-0.5 mL	Dry weighed: 200 mg

Note:

Please prepare enough dry ice or ice packs to ensure low temperature during sample delivery.
Our service is for research use only, and not for use in diagnostic procedures.
Plasma sample measurement requires at least 10 µl of plasma.

Key Features

High-throughput, and can identify up to 150 selected proteins at a time
High accuracy, specificity and repeatability, and can identify low abundance proteins
Broad dynamic range, covering 6 orders of magnitude of protein abundance at a time
Absolute quantification of a variety of proteins without the need for antibodies
No antibodies

Applications

Biomarker discovery and validation
Absolute quantitation: Absolute quantitation of proteins, peptides, metabolites and serum, lipids from plasma and other biological samples
Protein post-translational modification analysis
Verification of untargeted proteomics results
Pathological study of diseases

Reports

Experiment report
Relative parameters of the mass spectrometer
MS raw data files
Bioinformatics analysis
Peptide/Protein identification and intensity

* For Research Use Only. Not for use in the treatment or diagnosis of disease.

ICON 4D Proteomics with Data-Independent Acquisition (DIA)

50% detection rate of low-peak intensity proteins
Higher integrity and reproducibility of protein data
Nearly 100% ion utilization, greatly improving detection sensitivity
Fewer injections, shorter gradients, and deeper coverage
Stronger support for PTM identification

Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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Germany