Direct DIA Service

Direct DIA (direct Data-independent acquisition), also known as DDA-free DIA (dDIA), compared with the traditional DIA analysis strategy, does not need to carry out DDA hierarchical library construction and utilizes machine deep learning to achieve direct library generation by searching the DIA original file spectra. Deep learning scoring to find the pattern of spectral peak fragmentation, predict retention time, and remove false positive results can greatly improve efficiency and reduce cost, retaining the advantage of reproducible quantification of DIA, and the number of proteins identified is getting smaller and smaller with traditional DIA. In addition, Direct DIA and LFQ have the same technical advantages as DIA, and the number of dDIA proteins is the same as that of DIA and higher than that of LFQ. Creative Proteomics leverage the power of Direct DIA to provide a wide range of services that open new doors to understanding the proteome.

Advantages of Direct DIA

• Complete Coverage of the Proteome. The unmatched proteome coverage that direct DIA can provide is one of its main advantages. In contrast to conventional data-dependent acquisition (DDA) techniques, DIA methodically fragments and measures every peptide that can be identified within a sample. This leads to a more complete picture of the proteome, which includes post-translational modifications and low-abundance proteins.
• Quantitative Accuracy. Accurate protein and modification measurement is possible using direct DIA. Being highly accurate and reproducible, it's a useful tool for comparative and quantitative proteomic research. Proteins can be reliably identified and quantified by researchers with little bias.
• Reduced Missing Data. The issue of missing data, which arises frequently in DDA approaches, is lessened in DIA. As a result, the dataset becomes more trustworthy and researchers are able to base their choices on a comprehensive collection of protein identifications and quantifications.
• Enhanced Data Reproducibility. For large-scale initiatives and longterm investigations, the reproducibility of Direct DIA is essential. Because of its intrinsic consistency, data gathered throughout time or by multiple researchers is guaranteed to be extremely dependable and similar.
• Upgraded Targeted Proteomics. Targeted proteomics is another feature of direct DIA that helps scientists identify and measure particular proteins of interest in complicated samples. Because of this, it can be used in a variety of scientific applications.

Applications of Direct DIA

• Discovery of Biomarkers. The search for disease-related biomarkers, which enables early diagnosis and treatment response tracking, is made possible by direct DIA.
• Drug Research. DIA can be used in drug development to investigate the proteome effects of novel chemicals, which can help identify possible targets for drugs and comprehend how they work.
• Biology of Systems. Direct DIA is a tool that helps researchers understand biological systems by examining protein interactions, signaling networks, and pathways.
• Therapeutic Proteomics. Direct discriminant analysis (DIA) in clinical proteomics is a powerful tool for identifying disease-related protein biomarkers, forecasting patient outcomes, and developing customized therapy plans.
• Comparative Proteomics. The quantitative accuracy of Direct DIA is advantageous for comparative proteomic investigations including comparisons of samples under various circumstances or treatments.

Our Technical Advantages

• High Accuracy. High accuracy based on secondary ion quantification.
• High reproducibility. Few missing values in, low CV.
• High throughput. Several thousand proteins can be quantified off-label at the same time.

Technology Route

Why Choose Creative Proteomics?

• Expertise and Experience. Our team of highly qualified scientists and researchers has extensive experience in the industry and is knowledgeable with the subtleties of Direct DIA. We've demonstrated time and time again that we can get excellent results.
• State-of-the-Art Equipment. Modern mass spectrometry equipment and facilities are maintained by us, guaranteeing that your samples will be examined with the newest technology for the greatest outcomes.
• Customized Solutions. We are aware that every study endeavor is different. We collaborate closely with our clients to customize our Direct DIA services to meet their unique needs and ensure the information they receive aligns with their study goals.
• Data Analysis and Interpretation. We are more than just data generators. We offer thorough data interpretation and analysis, producing useful insights that advance your study.