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Data-Independent Acquisition (DIA) Proteomics

Accuracy: High-resolution mass spectrometry for precise results.
Stability: 1 ppm accuracy over time, minimal corrections.
Sensitivity: Detects low-abundance proteins (amol levels).
Efficiency: Monitors all proteins at once, faster analysis.
Speed: Rapid 12-20 Hz scanning.
Range: Wide dynamic range for comprehensive quantification.
Selectivity: Enhanced with msxDIA technology.

Reliable Identification: dia-PASEF enhances sensitivity and selectivity.
Speedier Analysis: TIMS separation accelerates while maintaining accuracy.
Comprehensive Coverage: Deeper insights with extensive proteome coverage.
High-Sensitivity Targeting: prm-PASEF boosts target monitoring without compromise.

MSX-DIA Quantitative Proteomics Services

Multiplexed data-independent acquisition (MSX-DIA), a variation of the DIA technique, is a new mass spectrometry technology put forward by MacCoss research group. In MSX-DIA, the mass range is divided into much narrower precursor isolation windows, each of which is usually about 4 m/z wide. At each scan cycle, the instrument randomly chooses N MS/MS of these windows containing precursor ions from which the MS/MS data can be obtained. This is repeated until the isolation windows list is empty. The non-negative least squares technique is used to assemble and demultiplex MS/MS spectra, thus eliminating the interference of the spectrum and providing higher selectivity than standard DIA. MSX-DIA technology, combined with Orbitrap mass spectrometer which has the advantages of ultra-high resolution (≥ 140,000), ultra-fast scanning speed (32-64ms) and ultra-high sensitivity (amol level), can collect all ion and fragment spectra without losing any information, with higher qualitative and quantitative accuracy for complex samples and well reproducibility. Besides, Skyline software is also used to manage the selection of isolation windows and process the data. MSX-DIA technology is able to analyze peptide sequences in the sample, select m/z peptides of interest, and support the quantitative analysis of thousands of polypeptides with high quantitative accuracy and consistency.

Workflow

MSX-DIA Workflow

Analytical Platform

AB SCIEX Triple-TOF 5600-plus, Q-Exactive, Orbitrap Fusion.

Bioinformatics Analysis

Problems to be Solved	Bioinformatics Analysis
Quality Assessment of Protein	DIA Data Analysis
Protein Comparison of Different Samples	Multivariate PCA Analysis
Protein Statistical Analysis	Venn Diagram
Protein Statistical Analysis	Volcano Plot
Functional Annotation	KEGG Annotation
	GO Annotation
	COG Annotation
Clustering Analysis	Hierarchical Clustering
Clustering Analysis	K-Means Clustering
Network Analysis	STRING Analysis

Key Features

Prioritize select some interested m/z peptides
High throughput, identify and quantitate thousands of proteins at once
High accuracy and repeatability
Quantitatively identify nearly all detectable proteins, including low abundance proteins
Pre-experiment method making is unnecessary

Applications

Large-scale quantitative analysis of protein
Biomarker discovery and validation
Target discovery
Mechanism and regulation mechanism research
Pathological study of disease
Genetic association study
Microorganisms proteomics research
Crop proteomics research

Sample Requirements

Based on our special protein extraction technology, we can quickly extract proteins from various samples and design personalized experimental schemes according to different experimental purposes. Specific requirements are as follows:

Sample Type	Protein	Cell	Animal Tissue	Plant Tissue	Blood	Urine	Serum	Microbes
Quantify	100 ug	1×10⁷ cells	1 g	200 mg	1 mL	2 mL	0.2-0.5 mL	Dry weighed: 200 mg

Note:

Please prepare enough dry ice or ice packs to ensure low temperature during sample delivery.
Our service is for research use only and is not intended for diagnosis.

Reports

Experiment report
Relative parameters of the mass spectrometer
MS raw data files
Bioinformatics analysis
Peptide/Protein identification and intensity

* For Research Use Only. Not for use in the treatment or diagnosis of disease.

ICON 4D Proteomics with Data-Independent Acquisition (DIA)

50% detection rate of low-peak intensity proteins
Higher integrity and reproducibility of protein data
Nearly 100% ion utilization, greatly improving detection sensitivity
Fewer injections, shorter gradients, and deeper coverage
Stronger support for PTM identification

Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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