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MSX-DIA Quantitative Proteomics Services

Multiplexed data-independent acquisition (MSX-DIA), a variation of the DIA technique, is a new mass spectrometry technology put forward by MacCoss research group. In MSX-DIA, the mass range is divided into much narrower precursor isolation windows, each of which is usually about 4 m/z wide. At each scan cycle, the instrument randomly chooses N MS/MS of these windows containing precursor ions from which the MS/MS data can be obtained. This is repeated until the isolation windows list is empty. The non-negative least squares technique is used to assemble and demultiplex MS/MS spectra, thus eliminating the interference of the spectrum and providing higher selectivity than standard DIA. MSX-DIA technology, combined with Orbitrap mass spectrometer which has the advantages of ultra-high resolution (≥ 140,000), ultra-fast scanning speed (32-64ms) and ultra-high sensitivity (amol level), can collect all ion and fragment spectra without losing any information, with higher qualitative and quantitative accuracy for complex samples and well reproducibility. Besides, Skyline software is also used to manage the selection of isolation windows and process the data. MSX-DIA technology is able to analyze peptide sequences in the sample, select m/z peptides of interest, and support the quantitative analysis of thousands of polypeptides with high quantitative accuracy and consistency.


MSX-DIA Workflow

Analytical Platform

AB SCIEX Triple-TOF 5600-plus, Q-Exactive, Orbitrap Fusion.

Bioinformatics Analysis

Problems to be SolvedBioinformatics Analysis
Quality Assessment of ProteinDIA Data Analysis
Protein Comparison of Different SamplesMultivariate PCA Analysis
Protein Statistical AnalysisVenn Diagram
Volcano Plot
Functional AnnotationKEGG Annotation
GO Annotation
COG Annotation
Clustering AnalysisHierarchical Clustering
K-Means Clustering
Network AnalysisSTRING Analysis

Key Features


Sample Requirements

Based on our special protein extraction technology, we can quickly extract proteins from various samples and design personalized experimental schemes according to different experimental purposes. Specific requirements are as follows:

Sample TypeProteinCellAnimal TissuePlant TissueBloodUrineSerumMicrobes
Quantify100 ug1×107 cells1 g200 mg1 mL2 mL0.2-0.5 mLDry weighed: 200 mg



* For Research Use Only. Not for use in the treatment or diagnosis of disease.

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Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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