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4D-DIA Quantitative Proteomics Services

4D-DIA proteomics is a next-generation DIA technology based on the timsTOFPro mass spectrometer for differential quantitative proteomics analysis. 4D-DIA proteomics scanning is faster and more sensitive compared to 3D-DIA technology. Meanwhile, the addition of the fourth-dimensional ion drip separation allows DIA data to be acquired without sacrificing window cycling speed while reducing spectral complexity and improving ion utilization, achieving a comprehensive improvement in proteomics in terms of coverage depth, sensitivity, and throughput.

Principle of DIA

Proteomics techniques can be divided into data-dependent acquisition (DDA) strategies and data-independent acquisition (DIA) strategies.

  1. DDA strategy: a limited number of peptide ions with higher intensities are acquired in each time window in the primary mass spectrometry and enter the secondary mass spectrum for fragmentation analysis.
  2. DIA strategy: the whole full scan range of the mass spectrum is divided into several windows and all ions in each window are selected, fragmented and detected cyclically.
  3. The DIA technique can obtain fragmentation information of all ions in the sample. Therefore, more quantitative information can be obtained, and the quantification is more accurate and reproducible based on the MS2 spectra.

4D-DIA Proteomics Services

Our 4D-DIA Proteomics Services:

Using timsTOF Pro, we have launched 4D-DIA proteomics services to help research in microsample proteomics, large cohort of clinical samples, and high-throughput protein analysis.



Data Analysis

Standard data analysis content
Statistical analysis of identification resultsSample protein and peptide identification histogram, PCA distribution map, quantitative variance statistical analysis
Bioinformatics analysisDifferential up- and down-regulated protein KEGG map, KEGG pathway function attribution, etc.
GO and G0 enrichment, KEGG and KEGG enrichment analysis, PPI interaction network and module analysis
Advanced data analysis content
Biomarker screeningIntegrated machine learning, LASSO regression analysis, marker panels, ROC analysis
Molecular typing analysisUnsupervised cluster typing analysis, proteome + transcriptome analysis, kinase analysis
Joint analysis of clinical characterization and omics results
Disease-related functional module analysisWGCNA co-expression analysis
Survival curve analysisClinical typing and survival curve analysis



Sample Requirements:

  1. Co-IP Samples: Protein requirement is around 2-5ug, do not include decontaminants (e.g. SDS) and high oxygen concentration in the sent protein samples; or you can send the beads containing protein samples to us directly and we will take care of the subsequent experiments.
  2. Tissue Samples: Please send us the tissue samples on dry ice.
  3. Tissue SamplesProtein# of CellsAnimal TissuePlant TissueBloodUrineSerumMicrobes
    Quantify100 ug1×107 cells>200 mg1 g1 mL2 mL0.2-0.5 mLDry weighed: 200 mg
  4. Protein Samples: Please make sure the total amount of protein is above 50ug; ordinary tissue and cell lysate can be used for protein extraction.
  5. Transportation: Please use sufficient amount of dry ice for transportation and try to use a fast postal delivery method to reduce the possibility of sample degradation during transportation.


In addition, we also provide 4D Label-Free Proteomics and 4D-PTMs Proteomics services. Please contact us for more details.

* For Research Use Only. Not for use in the treatment or diagnosis of disease.

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Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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