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Data-Independent Acquisition (DIA) Proteomics

Accuracy: High-resolution mass spectrometry for precise results.
Stability: 1 ppm accuracy over time, minimal corrections.
Sensitivity: Detects low-abundance proteins (amol levels).
Efficiency: Monitors all proteins at once, faster analysis.
Speed: Rapid 12-20 Hz scanning.
Range: Wide dynamic range for comprehensive quantification.
Selectivity: Enhanced with msxDIA technology.

Reliable Identification: dia-PASEF enhances sensitivity and selectivity.
Speedier Analysis: TIMS separation accelerates while maintaining accuracy.
Comprehensive Coverage: Deeper insights with extensive proteome coverage.
High-Sensitivity Targeting: prm-PASEF boosts target monitoring without compromise.

4D-DIA Quantitative Proteomics Services

4D-DIA proteomics is a next-generation DIA technology based on the timsTOFPro mass spectrometer for differential quantitative proteomics analysis. 4D-DIA proteomics scanning is faster and more sensitive compared to 3D-DIA technology. Meanwhile, the addition of the fourth-dimensional ion drip separation allows DIA data to be acquired without sacrificing window cycling speed while reducing spectral complexity and improving ion utilization, achieving a comprehensive improvement in proteomics in terms of coverage depth, sensitivity, and throughput.

Principle of DIA

Proteomics techniques can be divided into data-dependent acquisition (DDA) strategies and data-independent acquisition (DIA) strategies.

DDA strategy: a limited number of peptide ions with higher intensities are acquired in each time window in the primary mass spectrometry and enter the secondary mass spectrum for fragmentation analysis.
DIA strategy: the whole full scan range of the mass spectrum is divided into several windows and all ions in each window are selected, fragmented and detected cyclically.
The DIA technique can obtain fragmentation information of all ions in the sample. Therefore, more quantitative information can be obtained, and the quantification is more accurate and reproducible based on the MS2 spectra.

4D-DIA Proteomics Services

Our 4D-DIA Proteomics Services:

Using timsTOF Pro, we have launched 4D-DIA proteomics services to help research in microsample proteomics, large cohort of clinical samples, and high-throughput protein analysis.

Workflow

Data Analysis

Standard data analysis content
Statistical analysis of identification results	Sample protein and peptide identification histogram, PCA distribution map, quantitative variance statistical analysis
Bioinformatics analysis	Differential up- and down-regulated protein KEGG map, KEGG pathway function attribution, etc.
Bioinformatics analysis	GO and G0 enrichment, KEGG and KEGG enrichment analysis, PPI interaction network and module analysis
Advanced data analysis content
Biomarker screening	Integrated machine learning, LASSO regression analysis, marker panels, ROC analysis
Molecular typing analysis	Unsupervised cluster typing analysis, proteome + transcriptome analysis, kinase analysis
Joint analysis of clinical characterization and omics results
Disease-related functional module analysis	WGCNA co-expression analysis
Survival curve analysis	Clinical typing and survival curve analysis

Advantages:

Nearly 100% ion utilization rate greatly improves detection sensitivity
Significantly improve the detection depth
Comprehensively improve quantitative integrity

Application:

Larger cohorts of clinical sample analysis, such as precision typing of major diseases such as cancer and cardiovascular diseases.
The best way to detect clinical microsamples, such as: exosomes, FFPE paraffin sections, biopsy punctured tissues, etc.
Large-scale sample research on crop population traits, such as breeding lines, stress intervention, etc.
Clinical biomarker discovery: DIA is close to the quantitative ability of MRM, realizing the integration of marker screening and preliminary verification process.
Biological sample information database construction: DIA enables complete preservation of biological information for subsequent retrospective analysis such as drug response and protein expression profiling of gene knockout/overexpression.

Sample Requirements:

Co-IP Samples: Protein requirement is around 2-5ug, do not include decontaminants (e.g. SDS) and high oxygen concentration in the sent protein samples; or you can send the beads containing protein samples to us directly and we will take care of the subsequent experiments.
Tissue Samples: Please send us the tissue samples on dry ice.

Tissue Samples	Protein	# of Cells	Animal Tissue	Plant Tissue	Blood	Urine	Serum	Microbes
Quantify	100 ug	1×10⁷ cells	>200 mg	1 g	1 mL	2 mL	0.2-0.5 mL	Dry weighed: 200 mg

Protein Samples: Please make sure the total amount of protein is above 50ug; ordinary tissue and cell lysate can be used for protein extraction.
Transportation: Please use sufficient amount of dry ice for transportation and try to use a fast postal delivery method to reduce the possibility of sample degradation during transportation.

Report:

Experimental steps
Relevant experimental parameters
Mass spectrometry spectra
Raw data
Proteomics analysis results

In addition, we also provide 4D Label-Free Proteomics and 4D-PTMs Proteomics services. Please contact us for more details.

* For Research Use Only. Not for use in the treatment or diagnosis of disease.

ICON 4D Proteomics with Data-Independent Acquisition (DIA)

50% detection rate of low-peak intensity proteins
Higher integrity and reproducibility of protein data
Nearly 100% ion utilization, greatly improving detection sensitivity
Fewer injections, shorter gradients, and deeper coverage
Stronger support for PTM identification

Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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