Size Exclusion Chromatography Analysis Service

Creative Proteomics provides a size exclusion chromatography analysis service platform that can help you perform qualitative and quantitative analysis of the chemical properties of the detected substances.

Size exclusion chromatography (SEC) is the newest and most easily understood method of liquid chromatography. It is also called gel chromatography, gel filtration chromatography or gel permeation chromatography. It is the most effective method for quickly separating mixtures of different molecular weights. . Through the separation mechanism of molecular size exclusion, the gel, solvent molecules and polymer molecules with larger pore diameters are separated, and the chemical surface characteristics of polymer materials are detected. In the field of polymer research, molecular weight determination, solute diffusion theory and solute interaction theory research, material pore size and pore volume determination, solute classification and purification, and many other aspects have extremely important use value.

Principles of Size Exclusion Chromatography:

  • The size exclusion of molecules. In principle, there is no interaction between the sample components and the stationary phase. The stationary phase of the chromatographic column is a porous gel with different pore diameters, and only molecules with a critical diameter smaller than the opening of the gel pores are allowed to enter (retain ), its pore size is larger than that of solvent molecules, so solvent molecules can enter and exit freely.
  • Polymer molecules appear as random coils in the solution. The volume and molecular weight of the coils have a certain linear relationship. Solute molecules of different sizes can penetrate to different depths in gel pores of different sizes. Small solute molecules, Both large and small pores can go in, and can even penetrate into very deep pores. Therefore, small solute molecules have a long retention time and a large elution volume, while large solute molecules have a short retention time and a small elution volume.

Creative Proteomics can provide you with a size exclusion chromatography to analyze the relative molecular mass of macromolecules, analyze the molar mass of polymers, quantitatively analyze the molecular weight of organics, determine the molecular weight and distribution of macromolecules, purify biological macromolecules, analyze the degree of purification of crude oil and its heavy components.

Service We Can Provide:

  • Analyze molecules with high relative molecular mass such as proteins and nucleic acids
  • Analyze the molar mass of high polymer polyethylene and polyvinyl chloride
  • Quantitative analysis of the molecular weight of small and medium molecules and other organic substances
  • Determine the molecular weight and distribution of macromolecular peptides, proteins and polysaccharides
  • Purified biological large molecular weight
  • Analyze the degree of purification of crude oil and its heavy components

Advantages of Our Size Exclusion Chromatography Analysis Service:

  • Easy and feasible
  • Low sample volume required
  • Stable batch-to-batch reproducibility
  • High degree of automation
  • The recovery rate of protein is high, and the protein is not easy to inactivate
  • Can detect both hydrophobic protein and modified protein (PEGylated antibody)
  • Separate and prepare all conventional biological samples

Turnaround Time: 2-5 working days.

At Creative Proteomics, our professional analysts can provide you with improved quality services to support your business. Our professional analysis platform can guarantee consistently accurate and detailed delivery. Our professionals will customize exclusive solutions for you. Creative Proteomics looks forward to cooperating with you.

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