Subcellomics Creative Proteomics

Exosomes for mRNA Delivery

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Exosomes have emerged as promising carriers for mRNA delivery. Exosomes are natural transporters and therefore less toxic or immunoreactive. Moreover, their stability in the blood, their ability to cross biological barriers such as the blood-brain barrier (BBB), and their inherent targeting properties, make exosomes an ideal system for nucleic acid drug delivery. To promote the application of exosomes as carriers for therapeutical mRNA delivery, Creative Proteomics is committed to offering a series of exosome-related services, including exosome isolation, identification, labeling, manufacturing, and so on. With years of experience in exosome technology, we are confident to provide high-quality exosomes for global clients.

Fig. 1 mRNA-encapsulated exosomes.Fig. 1 mRNA-encapsulated exosomes. (Aslan, Cynthia, et al., 2021)

mRNA delivery system development

In vitro transcribed (IVT) mRNAs have recently shown great potential as a new drug class to deliver genetic information. Synthetically modified mRNAs provide the basics for a broad range of potential applications, including infectious disease vaccines for current and future pandemics, cancer immunotherapies, and the manufacturing of monoclonal antibodies. However, mRNA-based therapies face a variety of obstacles, including the large size of mRNA, inherent instability and high sensitivity to enzymatic degradation. In order to address the challenges of mRNA-based therapy, delivery systems are urgently needed for efficient mRNA delivery.

Continuous work is still necessary for further improvement of delivery systems to facilitate the wider application of mRNA-based therapy. To develop delivery systems that show a promising mRNA delivery profile, there are several key criteria that should be obeyed, including,

  • Reduce nonspecific uptake
  • Enhance tissue permeation
  • Targeted intracellular delivery
  • Controlled release kinetics

Support of exosomes as mRNA carriers

To use exosomes as a mRNA delivery system, sufficient quantities of exosomes must first be available. Moreover, exosomes must be pure enough to be free from cellular and molecular contamination. In order to describe the function of exosomes, it is necessary to carefully character their composition and carriers. Here, we are offering a series of exosome-related services for mRNA delivery, including,

Exosome analysis services
Exosome isolation and purificationSucrose gradient centrifugation
Polymer-based exosome enrichment method
Immunomagnetic bead method
Size exclusion chromatography method
Exosome identificationNanoparticle tracking analysis (NTA)
Electron microscopy analysis
Western blot
Exosome marker assayIsolation and enrichment of exosomal CD9, CD63, CD81, TSG101, HSP70 proteins
Exosome surface protein identification and quantitative analysis
Exosome engineeringExosome labeling and tracking
Cargo loading
Engineered exosome production
Cargo loading assessment
Exosomes labeling and trackingExosome fluorescent labeling
Fluorescence exosome purification
Fluorescent exosome concentration labeling
Exosome multiomics analysisExosome proteomics analysis:
Exosome protein profile identification
Exosome protein composition analysis
Exosome protein expression level analysis
Exosome protein differential expression analysis
Exosome metabolomics analysis:
Exosome differential metabolite screening
Qualitative and quantitative analysis of target metabolites/metabolic pathways
Exosome lipidomics analysis:
Exosome lipid composition and level analysis
Differential expression analysis of exosomal lipid molecules
Qualitative and quantitative analysis of targeted lipid molecules
Exosomal biogenesis and identificationOur capabilities will provide strong support for the study of exosome biogenesis and its identification.
Exosomal cargo and loading mechanismOur services can greatly help the study of exosomal cargo and loading mechanism, facilitating the diagnostic and therapeutic applications of exosomes.
Exosome function researchIn vitro analysis of the function of exosomes
In vivo analysis of the function of exosomes

Why choose us

  • Rich exosome research and analysis experience
  • Professional platforms with state-of-the-art technologies and equipment
  • Custom services according to our customers' samples and requirements
  • The best after-sales service

The recent advance in exosome technologies has greatly promoted the use of exosome-based mRNA delivery systems in the treatment of various diseases, such as breast cancers, Parkinson's disease, glioma, and leukemia. In addition to scalable upstream isolation, purification, and production of exosomes, we offer downstream processing for efficient exosome characterization and labeling to obtain high-quality, functional exosomes that can be used for mRNA delivery. If you are interested in our services, please don't hesitate to contact us. We will spare no effort to answer them for you.


  1. Aslan, Cynthia, et al. "Exosomes for mRNA delivery: A novel biotherapeutic strategy with hurdles and hope." BMC biotechnology 21.1 (2021): 1-12.
  2. Tsai, Shang Jui, et al. "Exosome-mediated mRNA Delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity." Journal of Biological Chemistry 297.5 (2021).

* For Research Use Only. Not for use in diagnostic procedures.

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