Two-dimensional gel electrophoresis (2-DE) is considered a powerful tool for proteomics work. It is used for separation and fractionation of
Membrane proteins are a class of proteins that interact with or are part of, biological membranes. Membrane proteins can be
The sequence of amino acids in a protein or peptide can be identified by Edman degradation, which was developed by
De novo is Latin which means “over again” or “anew”. The de novo peptide sequencing is a method for peptide
Proteomics studies play an increasing role in the field of biology. The use of mass spectrometry (MS) in combination with
Peptide Mass Fingerprinting (PMF), also known as mass fingerprinting, was developed in 1993. It is a high throughput protein identification
As we know before, the mass spectrometer consists of three main components, the ion source, the mass analyzer, and the
The mass spectrometer consists of three main components: the ion source, the mass analyzer, and the detector. In ion source,
Summary Cell metabolisms is usually defined as a collection of intracellular and membrane small molecule metabolites. Metabolite, which reflects the
Summary To find out protein-protein interaction is an advanced function of SILAC(stable isotope labeling with amino acids in cell culture)