Creative Proteomics is fervently dedicated to advancing science and innovation through our cutting-edge services in the structural analysis of DNA & RNA drugs. Backed by a team of expert scientists and state-of-the-art facilities, we offer comprehensive and dependable analyses, empowering our clients to fully realize the potential of their DNA & RNA drugs.

Introduction to DNA & RNA Drug Structural Characterization

Within the domain of pharmaceutical development, the structural analysis of DNA & RNA drugs holds profound significance. This intricate process entails meticulous examination of the drugs' chemical composition and conformational properties. Given that DNA & RNA drugs can vary in length and feature chemical modifications, gaining insights into their structural attributes becomes crucial for harnessing their complete therapeutic potential. Leveraging advanced technologies and techniques, such as nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and X-ray crystallography, this characterization furnishes intricate details about the three-dimensional structure of the drug, opening avenues for innovative and effective therapeutic solutions.

Fig. 1. NOESY spectrum for the aromatic base and sugar H2 region. (Spring-Connell A. M., et al., 2018)

Our DNA & RNA Drug Structural Characterization Services

Creative Proteomics offers a spectrum of DNA & RNA structural characterization services tailored to diverse research requirements.

Sequence Confirmation

Ensure the genuineness and precision of your DNA & RNA drug with our meticulous nucleotide sequence analysis. We employ advanced techniques to ascertain the exact sequence of bases, detect modifications, and identify any impurities, thereby ensuring the quality and efficacy of your drug candidate.

Secondary Structure Analysis

Rely on our extensive experience in secondary structure analysis to investigate the local folding patterns of your DNA & RNA drug, such as hairpins, loops, and stems. By deducing the secondary structure, we help you assess stability and binding properties, enabling you to make informed decisions during drug development.

Higher Order Structure Analysis

Gain a deeper understanding of your DNA & RNA drug with our higher-order structure analysis. We investigate the global folding and long-range interactions, which are vital for evaluating stability, resistance to degradation, and potential for aggregation. Our expertise in this area empowers you to optimize your drug candidate for maximum efficacy.

Equipped with state-of-the-art instrumentation and technologies, our laboratories are optimized for high-quality structural characterization services, delivering industry-leading outcomes. Below are the contents of our instruments and technologies.

• Nuclear Magnetic Resonance (NMR) Spectroscopy.
• X-ray Crystallography.
• Circular Dichroism (CD) Spectroscopy.
• Mass Spectrometry.
• Atomic Force Microscopy (AFM).

Process of Structural Characterization

The analysis of DNA & RNA higher-order structure is a meticulous and multi-step process.

Sample Preparation

High-quality samples are crucial for accurate analysis. DNA & RNA must be synthesized or isolated with care to avoid any structural modifications or degradation.

Choice of Analytical Techniques

Researchers must select appropriate techniques based on their specific research questions and the type of information required. NMR spectroscopy and X-ray crystallography are best suited for resolving atomic-level structures, while CD spectroscopy and mass spectrometry provide insights into higher-level structural features.

Data Acquisition

The selected techniques are then employed to obtain data from the DNA & RNA sample. This step requires expertise and precision, as the quality of data directly impacts the accuracy of the final structural model.

Data Analysis and Interpretation

The acquired data is then analyzed using specialized software and compared to known structures or theoretical models. The three-dimensional structure is interpreted, and any potential structural motifs or interactions are identified.

Validation

The proposed structural model is validated using multiple methods, such as re-analyzing the data, performing control experiments, or using complementary techniques. Validating the model enhances the confidence in the accuracy of the results.

DNA & RNA Drug Structural Characterization Application Fields

The characterization of DNA & RNA structures finds numerous applications across diverse fields.

• Drug Efficacy and Safety.
• Optimizing Formulation and Delivery.
• siRNA-based Therapeutics.
• mRNA Therapeutics.
• Antisense Therapies.

Creative Proteomics is passionate about helping our clients drive innovation in drug development through our exceptional services and scientific expertise. Trust us to be your premier partner on the journey to unlocking the full potential of your DNA & RNA therapeutics. Contact us today to discuss your DNA & RNA drug structural characterization needs.