At Creative Proteomics, we recognize the critical significance of mRNA characterization in unraveling the mysteries of biology and offer cutting-edge mRNA characterization services to catalyze advancements across diverse domains.

Introduction of mRNA Characterization

mRNA, also known as messenger ribonucleic acid, acts as an essential intermediary between DNA, the genetic blueprint, and proteins, the functional workhorses of cells. mRNA carries the genetic information encoded in DNA to the cellular machinery responsible for protein synthesis. This process, known as gene expression, drives cellular functions and ultimately dictates an organism's phenotype. mRNA characterization plays a pivotal role in deciphering the intricacies of gene expression. It involves the comprehensive analysis of mRNA molecules, including their structure, sequence, modifications, and abundance. This multifaceted approach offers researchers a deeper understanding of gene regulation, post-transcriptional modifications, and the interplay of various factors that influence mRNA stability and translation.

Fig. 1. Schematic illustration of mRNA sequence mapping workflow. (Vanhinsbergh CJ, et al., 2022)

Our mRNA Characterization Services

At Creative Proteomics, we take great pride in offering advanced services for characterizing mRNA. These services are designed to empower professionals working in academic, pharmaceutical, and biotechnology domains. Our comprehensive range of service options encompasses a wide array of techniques used for mRNA analysis. These services equip our clients with unmatched insights, enabling them to delve deeper into their research pursuits.

LC-MS/MS Analysis

We leverage sophisticated technology in the form of liquid chromatography-tandem mass spectrometry (LC-MS/MS) to provide an exhaustive comprehension of interactions between proteins and RNA, modifications within mRNA, and other pivotal molecular facets.

Structural Analysis

Our strategies incorporate leading-edge techniques aimed at unraveling the structural components of mRNA, encompassing secondary structures as well as interactions involving RNA and proteins. These revelations offer illumination on matters of mRNA stability and mechanisms that govern regulation.

Post-Transcriptional Modifications

Our services encompass the detection and analysis of various modifications on mRNA molecules, such as methylation and acetylation, which can influence mRNA localization, translation efficiency, and stability.

Quantitative Analysis

We provide quantitative data on mRNA abundance, allowing you to compare gene expression levels across different samples or conditions.

mRNA Drug Stability Studies

With state-of-the-art facilities and cutting-edge analytical techniques, we offer services to accurately monitor mRNA degradation, secondary structure changes and protein production levels. Whether it is an initial stability assessment or an in-depth long-term study, our services are tailored to meet the specific needs of our clients.

mRNA Translation Efficiency Evaluation

Our comprehensive range of services encompasses the evaluation of mRNA translation dynamics. Leveraging advanced methodologies and our proficient acumen, we are adept at tailoring solutions to align with your research requisites.

Transcriptome Profiling

Utilizing methodologies like RNA-Seq, we furnish meticulous insights into the transcriptome, the complete collection of mRNA molecules within a cell. This facilitates the identification of genes exhibiting differential expression, events of alternative splicing, and the discovery of novel transcripts.

Functional Insights

By integrating mRNA characterization data with functional annotations, we assist in elucidating the biological roles of specific mRNA molecules.

Workflow of mRNA Characterization Services

Our workflow is meticulously designed to ensure comprehensive and accurate mRNA characterization. It encompasses several key steps.

Sample Preparation

We start by isolating high-quality mRNA from your samples, ensuring that the downstream analysis is built on a solid foundation.

Assay Design & Optimization

Our experienced scientists carefully design and optimize assays specific to the mRNA.

Characterization & Analysis

We utilize cutting-edge instrumentation, such as LC-MS/MS and LC-HRAM systems, to perform mRNA characterization.

Data Analysis and Reporting

We provide comprehensive reports, summarizing the results and ensuring a clear interpretation of mRNA characterization analysis outcomes.

mRNA Characterization Application Fields

The applications of our mRNA characterization services are vast and impactful, spanning a wide range of research and development areas.

• Basic Research.
• Disease Mechanisms.
• Drug Discovery.
• Vaccine Development.
• Personalized Medicine.

At Creative Proteomics, we believe that unlocking the potential of mRNA characterization is pivotal for driving breakthroughs in biological research and therapeutic innovation. Whether you're delving into the intricacies of gene regulation or seeking novel therapeutic avenues, our mRNA characterization services are designed to accelerate your journey. Contact us today to explore how our advanced mRNA characterization services can empower your research and elevate your scientific endeavors to new heights.