Creative Proteomics offers a wide range of cutting-edge analytical services to support your research and development needs. Our expertise spans across diverse fields, and we are excited to introduce our state-of-the-art DNA & RNA size and aggregation analysis services.

Introduction of DNA & RNA Drug Size and Aggregation Analysis

DNA & RNA size and aggregation analysis is a scientific process that evaluates the size distribution and aggregation propensity of DNA & RNA drugs. The technique involves characterizing these biomolecules based on their length, shape, and the extent of aggregation in a given sample. In the context of therapeutic DNA & RNA drugs, such as antisense oligonucleotides, siRNAs, and aptamers, understanding their size and aggregation behavior is crucial for ensuring their safety, efficacy, and stability during development and subsequent usage.

Fig. 1. Characterization of D-17 and D-17PG micelles by atomic force (AFM) and transmission electron (TEM). (Pavlova A, et al., 2020)

Our DNA & RNA Size and Aggregation Analysis Services

Creative Proteomics offers professional DNA & RNA drug size and aggregation analysis services. We employ state-of-the-art equipment and techniques to provide comprehensive insights into DNA & RNA drug behavior. There are some key services.

Dynamic Light Scattering (DLS)

Our DLS service determines the hydrodynamic size and aggregation propensity of your DNA & RNA drug samples in solution. This non-invasive technique provides critical insights into their behavior and stability.

Microscopy

Through atomic force microscopy (AFM) and transmission electron microscopy (TEM), we offer high-resolution imaging of DNA & RNA drugs, giving you a deeper understanding of their structural characteristics and aggregation patterns.

Analytical Size-Exclusion Chromatography (SEC)

Our SEC service separates DNA & RNA drugs based on their size, enabling the quantification of aggregates and impurities within your samples.

Electrophoresis Techniques

Our electrophoresis services, including gel electrophoresis and capillary electrophoresis, allow for the separation and analysis of DNA & RNA drugs based on their size and charge, providing valuable qualitative and quantitative information.

Analytical Ultracentrifugation (AUC)

With our AUC service, we precisely measure the molecular weight and shape of your DNA & RNA drugs by analyzing their sedimentation velocity or equilibrium. This technique is ideal for complex samples and aggregates.

Process of DNA & RNA Drug Size and Aggregation Analysis

The DNA & RNA size and aggregation analysis process involves several steps to obtain accurate and reliable data. There are general processes.

Sample Preparation

The first step involves preparing the DNA & RNA sample in the appropriate buffer and concentration to mimic its intended application or physiological conditions.

Instrumentation and Analysis

The prepared sample is then subjected to the chosen analytical techniques, such as DLS, AUC, electrophoresis, or SEC. The instruments generate data based on the interactions between the DNA & RNA drugs and the measurement principle.

Data Interpretation

Expert analysis and software algorithms are used to interpret the data generated by the instruments. This step involves understanding the size distribution, aggregation propensity, and any other relevant information.

Report Generation

Finally, a comprehensive report is generated, detailing the findings of the analysis. The report provides crucial information for researchers, manufacturers, and regulatory authorities.

Size and Aggregation Analysis Application Fields

DNA & RNA size and aggregation analysis finds applications in various fields, ranging from basic research to drug development and beyond.

• Quality Control and Characterization.
• Formulation Development.
• Safety Assessment.
• Regulatory Compliance.
• Nanomedicine and Drug Delivery.
• Therapeutic Oligonucleotides.
• Diagnostics and Biomarker Discovery.

At Creative Proteomics, we are committed to enhancing your research with our specialized services in DNA & RNA drug size and aggregation analysis. Our team of experts and cutting-edge innovations are here to support your scientific exploration, driving you toward groundbreaking discoveries. If you would like to learn more about our services, kindly contact us for comprehensive information.

Reference

1. Pavlova A; et al. (2020). Amphiphilic "Like-a-Brush" Oligonucleotide Conjugates with Three Dodecyl Chains: Self-Assembly Features of Novel Scaffold Compounds for Nucleic Acids Delivery. Nanomaterials (Basel.). 10(10):1948.