Introduction of siRNA Specificity

Small interfering RNA (siRNA) is a class of double-stranded RNA molecules that play a pivotal role in the post-transcriptional gene silencing process. These molecules hold great promise as therapeutic agents by selectively inhibiting the expression of disease-related genes. siRNA specificity refers to the accuracy and precision with which these molecules target the intended gene sequence while minimizing unintended off-target effects on other genes. Achieving high specificity is a paramount objective to maximize therapeutic benefits and minimize potential adverse effects.

Fig. 1. Schematic representation of the potential problems that can arise during RNAi experiments that use synthetic siRNA duplexes. (Cullen B. R., 2006)

Methods of siRNA Specificity Determination

High-throughput sequencing techniques enable the identification of potential off-target effects by comparing the transcriptomes of cells treated with siRNA to those of untreated cells. Computational analysis further aids in predicting off-target sites based on sequence homology. Functional assays, such as reporter gene assays, confirm the impact of potential off-target effects on gene expression. These methods collectively provide a comprehensive picture of siRNA specificity.

Applications of siRNA Specificity Determination

The applications of siRNA specificity determination are vast and impactful.

• Optimization of siRNA delivery methods.
• Drug Development.
• Optimization of Dosing Regimens.
• Reducing the Risk of Toxicity.
• Confirming the Specificity of Knockdown Effects.

What We Provide?

At Creative Proteomics, we offer state-of-the-art siRNA specificity determination services to support your RNA-based therapeutic endeavors. Our expert team combines cutting-edge technologies with extensive experience to provide accurate and reliable results.

Quantitative Analysis

We provide quantitative analysis services for your various molecules to ensure that the concentration of siRNA molecules used in your experiments is accurate, thus avoiding non-specific effects.

Potency Assessment

We provide accurate potency testing to assess the potency of your siRNA molecules to ensure that they can specifically affect the target gene at the desired concentration.

Sequence Confirmation of DNA & RNA

We provide a comprehensive sequence confirmation service to confirm the sequence of your siRNA molecules to ensure that they match the designed specific sequence.

Forced Degradation Studies

We conduct thorough stability investigations that replicate the degradation of siRNA molecules under conditions of stress, including scenarios involving elevated temperatures and oxidative environments. The primary objective is to evaluate the resilience of siRNAs and the potential formation of degradation byproducts.

DNA & RNA Drug Identification

We provide drug identification services, our services analyze samples to be able to determine if the desired siRNA drug molecule is present in them to ensure the correct reagent substance.

Conclusions

In the rapidly evolving landscape of RNA-based therapeutics, siRNA specificity determination stands as a critical determinant of success. Accurate characterization of siRNA specificity enhances therapeutic efficacy, reduces adverse effects, and accelerates the development of innovative treatments.

At Creative Proteomics, our advanced techniques and comprehensive services empower you to confidently progress your siRNA-based therapeutic projects. Ready to enhance the specificity of your siRNA molecules? Contact us today to learn more about our siRNA specificity determination services.

Our Advantages

• Our team of scientists boasts extensive knowledge in RNA-based therapeutics, ensuring the accuracy and reliability of our services.
• We leverage the latest high-throughput sequencing and bioinformatics tools to provide a comprehensive analysis of siRNA specificity.
• We understand that each project is unique. Our services are tailored to meet your specific needs, ensuring the most relevant results.
• We prioritize efficiency without compromising quality, delivering results within agreed-upon timelines.
• We consider our clients as partners. We work closely with you to understand your goals and provide insights that drive project success.