Introduction of siRNA Delivery Efficiency

siRNA, a revolutionary tool in molecular biology, plays a pivotal role in gene silencing by targeting specific mRNA molecules for degradation. However, its effectiveness hinges on successful delivery to the target cells. The term siRNA delivery efficiency refers to the extent to which siRNA molecules are transported to and taken up by the intended cells. The success of siRNA-based therapies greatly depends on efficient delivery to the target tissues or cells. Poor delivery efficiency can lead to inadequate gene silencing, potentially undermining the therapeutic outcomes. By assessing siRNA delivery efficiency, researchers and pharmaceutical developers can refine delivery systems and enhance treatment efficacy while minimizing off-target effects.

Fig. 1. Challenges related with in vivo small interfering RNA (siRNA) delivery. (Serrano-Sevilla I., et al., 2019)

Types of siRNA Delivery Systems

There is a range of delivery systems available to facilitate the transportation of siRNA molecules into cells. These systems can be broadly categorized into viral and non-viral delivery methods.

What We Provide?

At Creative Proteomics, we are at the forefront of providing comprehensive siRNA delivery efficiency assessment services. Our state-of-the-art facilities and expert scientists enable us to offer a wide range of assays to accurately evaluate the success of siRNA delivery. Whether you are developing novel delivery systems, conducting preclinical studies, or optimizing therapeutic strategies, our services are tailored to meet your specific needs.

Quantitative Analysis

We offer a comprehensive quantitative service that allows for the assessment of siRNA delivery efficiency by measuring the expression levels of target genes. If successful delivery results in a significant decrease in the expression of the target gene, this indicates high delivery efficiency.

Potency Assessment

We provide potency testing services, which can assess the potential of delivery efficiency by measuring the silencing effect of specific genes triggered by siRNA after delivery. A higher silencing effect indicates a higher delivery efficiency.

Size and Aggregation Analysis

We provide professional size and aggregation analysis, by analyzing the size and aggregation status of the siRNA delivery system, we can understand its distribution and delivery efficiency in vivo.

Encapsulation Efficiency (LNP) Determination

We provide Encapsulation Efficiency analysis, which can be performed on liposome nanoparticle (LNP) vectors or virus-like particles (VLP) vectors, and can reveal the distribution of siRNA in the vectors, which affects the delivery efficiency.

Conclusions

siRNA delivery efficiency assessment stands as a cornerstone in the realm of genetic therapies and molecular research. By precisely determining how effectively siRNA molecules are delivered to target cells, researchers can refine and optimize delivery systems, paving the way for innovative treatments and scientific breakthroughs.

At Creative Proteomics, we are dedicated to partnering with you to achieve enhanced siRNA delivery and transformative advancements in biomedicine. Ready to elevate your siRNA delivery efficiency assessment? Contact us today to learn more about our specialized services and how we can assist you in achieving your research and therapeutic goals.

Our Advantages

• Our team comprises experienced scientists with a deep understanding of siRNA delivery mechanisms and assessment techniques.
• We employ the latest technologies and methodologies to ensure accurate and reliable results.
• Our services are tailored to your unique requirements, ensuring that you receive the most relevant data for your project.
• We understand the importance of time in research and provide timely results to keep your projects on track.
• Your research and data security are our top priorities. We maintain strict confidentiality throughout the process.
• We cater to clients worldwide, facilitating international collaborations and knowledge exchange.