Introduction of mRNA and siRNA Molecules

Messenger RNA (mRNA) and small interfering RNA (siRNA) are vital RNA molecules with crucial roles in gene expression regulation. mRNA acts as a messenger, transcribing genetic information from DNA and delivering it to ribosomes for protein synthesis. On the other hand, siRNA plays a key role in RNA interference (RNAi), silencing target genes to control gene expression. Analyzing these molecules helps uncover their impact on disease development, gene regulation, and therapeutic potential. Comprehensive analysis of mRNA and siRNA molecules aids in identifying potential drug targets and developing innovative RNA-based therapies.

Fig. 1. Gene silencing mechanisms of siRNA and miRNA. (Lam J. K., et al., 2015)

Types of mRNA and siRNA molecules

• mRNA

Different types of mRNA molecules exist, including precursor mRNA (pre-mRNA), mature mRNA, alternative splicing variants, and non-coding RNAs (ncRNAs).

• siRNA

siRNA molecules can be endogenous or exogenous. Endogenous siRNAs are produced within cells and play a critical role in gene silencing, while exogenous siRNAs are synthetic molecules introduced into cells to target specific genes.

Applications of mRNA and siRNA Molecules

Disease Mechanism Research

Investigating mRNA and siRNA expression profiles can unravel the molecular basis of diseases, leading to potential therapeutic targets.

Drug Development

mRNA and siRNA molecules serve as potential drug candidates or targets for developing innovative therapies, including RNA-based therapeutics.

Cancer Research

Identifying aberrantly expressed mRNA and siRNA molecules can aid in understanding oncogenic processes and facilitate the development of targeted therapies.

Infectious Diseases

Studying the interplay between host mRNA and viral siRNA can reveal insights into host-pathogen interactions and antiviral strategies.

What We Provide?

Creative Proteomics offers a comprehensive suite of services for mRNA and siRNA molecules analysis, tailored to meet the specific needs of our clients.

Sequence Confirmation

We offer a complete sequence confirmation service to verify the sequence accuracy of DNA or RNA samples. For specific sequences of RNA molecules such as mRNA or siRNA, we provide sequence confirmation by Sanger sequencing and other technical methods.

Secondary Structures Analysis

At Creative Proteomics, we provide effective services for analyzing the secondary structures of nucleic acid molecules. Our advanced instrumentation allows for a thorough examination of the secondary structure of genetic material, utilizing methods such as NMR or circular dichroism.

Quantitative Analysis

Our services in quantitative assessment aid in determining the concentration of specific molecules within samples containing genetic material. Utilizing techniques like qPCR, we can precisely quantify the abundance of distinct mRNA or siRNA sequences.

Impurity Profiling of DNA and RNA Drugs

We extend a comprehensive array of services for analyzing impurities, aiming to assist in the detection and evaluation of foreign components within pharmaceutical samples. This support facilitates drug development efforts and upholds the quality of pharmaceutical products. By utilizing techniques such as mass spectrometry and nuclear magnetic resonance (NMR), we can deduce the structure and composition of diverse impurities.

Conclusions

In conclusion, mRNA and siRNA molecules analysis plays a crucial role in advancing biomedical research and holds immense potential in the development of personalized medicine and therapeutic interventions.

Creative Proteomics is committed to providing state-of-the-art services to support researchers worldwide in their quest to unravel the complexities of gene expression regulation and pave the way for groundbreaking discoveries. For inquiries and more information about our mRNA and siRNA molecules analysis services, please feel free to contact us.

Our Advantages

• We utilize the latest techniques for accurate and efficient analysis.
• Our experienced scientists are dedicated to delivering high-quality results.
• We tailor our services to meet the unique requirements of each project.
• We understand the importance of deadlines and ensure on-time delivery of results.
• We prioritize the confidentiality of all client data and research.