Metabolic Flux Analysis Solution

About the Infographics

Unlock the dynamics of cellular metabolism with our advanced Metabolic Flux Analysis (MFA) platform. Combining stable isotope labeling with high-resolution mass spectrometry and robust computational modeling, our solution provides real-time, pathway-specific insight into metabolic activity. MFA moves beyond static metabolite profiles to uncover actual metabolic rates—enabling researchers to trace fluxes, identify bottlenecks, and optimize metabolic engineering strategies across diverse biological systems.

Key Features

• Stable Isotope Labeling: Precision tracking of ¹³C-, ¹⁵N-labeled substrates in live systems.
• HRMS, LC-MS/MS & GC-MS: High-resolution detection of isotopomers and metabolic intermediates.
• Quantitative & Qualitative Flux Analysis: From pattern identification (MID) to absolute flux rate calculations.
• Custom Tracer Design: Targeted studies using tailored isotope tracers for pathway-specific applications.

Service Highlights

• Wide Pathway Coverage: Glycolysis, TCA, PPP, amino acid, fatty acid, and nucleotide metabolism.
• Multiple MFA Types: Including ¹³C-glucose MFA, ¹³C/¹⁵N-glutamine MFA, INST-MFA, dynamic flux studies, and dual-tracer analysis.
• Advanced Computational Modeling: Use of INCA, 13CFLUX2, and OpenFLUX for robust flux estimations and biological interpretation.
• In Vivo Expertise: Support for flux tracing in mouse models and compartmentalized metabolism (e.g., mitochondrial flux).
• High-Quality Standards: GLP compliance, expert model validation, and strict QC procedures ensure reliable results.

You may be interested in:

• Glycolysis
• Pentose Phosphate Pathway
• TCA Cycle
• Amino Acid Metabolism
• Fatty Acid Metabolism
• One-Carbon Metabolism
• Nucleotide Metabolism