Creative Proteomics offers its customers end-to-end solutions for protein gel services including SDS-PAGE, IEF and native PAGE analysis. Polyacrylamide gel electrophoresis (PAGE) is one of the most frequently employed techniques for separating macromolecules including DNA, RNA, and proteins
In 1-D electrophoresis, the proteins separated in one dimension will lie along a lane, and then the molecules are spread out across in the 2-D gel. Isoelectric focusing (IEF) and Sodium Dodecyl Sulfate Poly Acrylamide Gel Electrophoresis (SDS-PAGE) are preferred in 2-DE separation.
In Western Blot analysis, the proteins of the sample are first separated using various methods including SDS-PAGE and IEF. After the gel electrophoresis analysis, the proteins are moved from within the gel onto a membrane made of nitrocellulose or polyvinylidenedifluoride ...
In biological membranes, many proteins are organized in complexes. Creative Proteomics offers a method for the global analysis of the subunits of these protein complexes through 2D Blue Native / SDS-PAGE analysis. In 2D BN / SDS-PAGE analysis, the samples were analyzed in the 1st dimension by blue native polyacrylamide gel electrophoresis (BN-PAGE), and then separated by SDS-PAGE in the 2nd dimension which is 90 degrees from the first.
This value of surface charge is useful for understanding and predicting interactions between particles in suspension. Creative Proteomics offers a service platform for the estimation of protein isoelectric point.
The evaluation of the spot patterns is the most demanding part of the 2-D electrophoresis technique, and the images would be analyzed comparatively and automatically. 2-D gel patterns are compared to detect qualitative and/or quantitative protein ex pression changes between individual samples or different populations. Imaging analysis of 2-D gels can provide various types of information for the detection of novel, missing, or modified proteins; quantification of protein spots; determine of pI and Mr values of protein spots; enzyme digest and mass spectrometry detection.