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Proteomics Service

De novo protein/peptides sequencing service

The sequence of peptide/protein is important to study the biological function of the peptide/protein. However, complete characterization of peptides/proteins, including post-translational modifications (PTMs), sequence mutations and variants, is very challenging. There are two approaches to determine the sequence of peptide/protein by mass spectrometry: database search and de novo sequencing. Database search approach compares acquired mass spectra to a database of known protein sequences to identify the protein sequences. De novo sequencing is a process in which amino acid sequences are directly interpret from tandem mass spectra without the assistance of a database.

Peptide/Protein De Novo Sequencing

Although database search identification of proteins by mass spectrometry is well established, the methods do not apply if the protein sequence does not exist in the current database, therefore, de novo sequencing is the only method for identifying novel peptides, unsequenced organisms, and antibodies drugs, which database search methods were not able to detect. However, De novo sequencing poses more challenging than the traditional database search approach, such as, ambiguous assignments of fragment ions, incomplete fragmentation generate insufficient product ions leading to low sequence coverage and difficulty in distinguishing ion series, notably N-terminal from C-terminal MS/MS product ions (b ions from y ions).

In Creative Proteomics, we tackle these challenges by using the following four strategies. Firstly, de novo sequencing is prone to false positives partly due to low mass resolution and accuracy. Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) has the highest mass resolution and accuracy. With 7T solariX XR FTICR-MS, the mass resolving power can research 10,000,000. As the mass accuracy increases, the resulting assignments become increasing confident. Secondly, both bottom-up and top-down mass spectrometry are used to analyze the same sample, bottom-up allow us to use different enzyme digestion to generate overlapping peptides while top-down mass spectrometry offers intact mass data and provide protein fragmentation details. Combine these data can better assemble and confirm the protein sequence. Thirdly, four types of fragmentation techniques: collision induced dissociation (CID), electron transfer dissociation (ETD), electron capture dissociation (ECD) and high energy collisional dissociation (HCD) are employed for peptide/ protein fragmentation, which could provide more fragment ions from the same peptide/protein and these complementarity data will ensure to improve the ion assignment. For example, it would be possible to distinguish C-terminal (z•) from N-terminal (c’) ECD product ions based on the ratio of prime to radical ion abundance in ECD vs activated-ion ECD (AI-ECD) MS/MS product ion mass spectra. Finally, we also provide chemically derivation to identify ion series. For example, introduce bromide on the C-terminus by oxazolone chemistry can enable identification of y ions because of the distinct bromide isotope peaks. Similarity, implement guanidination can increases selectivity and identification.

Technology platform

  • Nano HPLC- 7T solariX XR FTICR-MS (equipped with ECD)
  • Nano HPLC- Orbitrap Fusion™ Lumos™ Tribrid™ MS (equipped with ETD)

Sample Requirement

  • Normal amount: 500ug/protein
  • Minimum amount: 200ug/protein

Summary

  • Determine the sequence of protein by HPLC-MS.

Report

  • A detailed technical report will be provided at the end of the whole project, including the sample preparation, HPLC-MS/MS parameters.
  • The sequence (FDR<1%) of the protein, molecular weight will be reported in the report.

Ordering Procedure:

Ordering Procedure


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