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Overview of Metabolomics Services

Metabolomics is the study of metabolism, specifically the science of identifying and quantifying the biochemical byproducts of metabolism, called cellular metabolites. This is achieved by using analytical technologies such as NMR and mass spectrometry combined with sophisticated statistical methods to interpret the generated data. Compared with genomics, transcriptomics and proteomics, metabolomics provide a direct and global snapshot of all the metabolites, and tell the researchers what have happened, making it more and more popular in disease research, toxicology, environmental analysis, agriculture, biofuel development and nutrition.

Creative Proteomics can help you to deal with various formats of biological samples of different origins, such as biological fluids, tissues, and food products. All the analytical procedures are performed with advanced NMR and Mass spectrometry instruments. Especially the MS platform, combined with different MS detector (TOF, quadrupole and ion trap, or tandem/hybrid MS), gas chromatography (GC) or liquid chromatography (LC).

Our metabolomics services include:

  • Global metabolite profiling
  • Targeted & untargeted metabolomic profiling
  • Lipidomic profiling
  • Metabolite identification & quantification

Applications of our services:

  • Biomedical area: metabolomic profiling of biological fluids, such as urine, blood, and serum
  • Pharmaceutical area: drug toxicity analysis
  • Food: fruits, vegetables, dairy products, olive oil, etc.

Advantage of our service:

  • Rapid and reliable analytical results
  • Easy to read report
  • Identification and quantification of several classes of metabolites in several species
  • Lipidomic profiling platform across several lipid classes
  • Quantification of polar and non-polar metabolites with a quality control system

Overview of our metabolomics services:

Application Platforms Compounds Sample Preparation Sample Type Numbers of Detected Compounds
Untargeted Metabolomics (Biomarker) GC-MS polar compounds(organic acids, carbohydrates, amino acids, fatty acids, polyhydric alcohols, phosphoric acids and derivatives) ultrasonic extraction, LLE, nitrogen sample concentration, derivative plant ~100
organism
body fluid
cell
tissue
fatty acids rotary evaporation, Soxhlet extraction, esterification reaction plant 10-30
organism
body fluid
cell
tissue
volatile/aromatic compounds (organic acids, alkanes, phenols, alkene, alcohols, aldehydes, esters, ketones, amides, etc.) organic solvent, simultaneous distillation, steam distillation, Soxhlet extraction, Bligh-Dyer method plant dozens-hundreds
organism
body fluid
cell
tissue
LC-MS(Q-TOF) conventional metabolomics grinding, sonication plant 200-400 Peaks
cell crush, LLE organism
centrifugation, precipitation body fluid
cell crush, LLE cell
homogenate, LLE tissue
lipidomics Bligh-Dyer, MTBE faces detect PC/LysoPC, PE/LysoPE simultaneously
plant
organism
body fluid
cell
tissue
Targeted Metabolomics GC-MS fatty acids 37 fatty acids    
carbohydrate fructose, glucose, etc.    
LC-MS/MS organic acids acetic, malic acid, etc.    
amino acids over 20    
polyamines 13    

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